基于APTLD的猪肉中莱克多巴胺残留含量的三维同步荧光光谱快速测定  

Rapid Determination of Ractopamine Content in Pork by Using Three-Dimensional Synchronous Fluorescence Spectrum Coupled with APTLD

作　　者：赵进辉[1] 袁海超[1] 刘木华[1] 肖海斌[1] 洪茜[1] 

机构地区：[1]江西农业大学工学院/生物光电及应用重点实验室,江西南昌330045

出　　处：《光谱学与光谱分析》2014年第4期1012-1016,共5页Spectroscopy and Spectral Analysis

基　　金：国家科技支撑计划课题项目(2012BAK17B02);国家自然科学基金项目(31101295);江西省科技厅对外科技合作计划项目(20132BDH80005);江西省科技厅科技支撑项目(20121BBG70058);江西省教育厅科技计划项目(GJJ12244))资助

摘　　要：应用三维同步荧光光谱法结合交替惩罚三线性分解(APTLD)来建立猪肉中莱克多巴胺残留含量的定量测定模型,以实现猪肉中莱克多巴胺残留含量的快速测定。首先分析了莱克多巴胺的荧光光谱产生机理和样本的三维同步荧光光谱;其次对猪肉提取液中的莱克多巴胺荧光的浓度猝灭现象进行了分析;然后应用核一致诊断法确定了APTLD的三线性分解组分数为2,并建立了猪肉提取液中莱克多巴胺的相对荧光峰值强度与训练样本中莱克多巴胺的相对荧光峰值强度之间的标定曲线,用于待测样本中的相对荧光峰值强度的校正;最后,建立了基于APTLD的猪肉中莱克多巴胺残留含量的三维同步荧光光谱预测模型。试验结果表明,该方法可以较好的解决猪肉样本中莱克多巴胺与背景之间的同步荧光光谱严重重叠的问题,省去了一些烦琐的"化学分离"过程,模型预测集的决定系数(R^2)和均方根误差(RMSEP)分别为0.986 3和0.496 6 mg·L^(-1),达到了猪肉中莱克多巴胺残留含量快速定量测定目的。In order to realize the rapid determination of ractopamine content in pork ,quantitative determination model of racto-pamine content in pork was established by using three-dimensional synchronous fluorescence spectrum coupled with alternating penalty trilinear decomposition （APTLD） .Firstly ,the generation mechanism of the fluorescence spectrum for ractopamine and three-dimensional synchronous fluorescence spectrum for samples were analyzed .Secondly ,concentration quenching phenome-non of fluorescence of ractopamine in pork extract was investigated .Thirdly ,the number of components for three linear decom-position of APTLD was set as 2 by using the core consistency diagnostic method ,and the calibration curve of the relative fluores-cence intensity of ractopamine between pork extract and the training sample was established for the correction of relative fluores-cence intensity of prediction samples .Finally ,three-dimensional synchronous fluorescence spectrum combined with APTLD was used to build the prediction model of ractopamine content in pork .The experimental results showed that the method adopted in the paper could better solve the problem of serious synchronous fluorescence spectrum overlapping between ractopamine in pork samples and backgrounds ,and leave out some trivial process of chemical separation for the identification of ractopamine in pork . The determination coefficient （R2 ） and the root mean squared error of prediction （RMSEP） for the model proposed in this paper were 0 .986 3 and 0 .496 6 mg ＆#183; L -1 ,respectively .The method in this paper has achieved the goal of rapid quantitative detection of ractopamine content in pork .

关 键 词：交替惩罚三线性分解 三维同步荧光光谱 莱克多巴胺 猪肉 ALTERNATING PENALTY trilinear DECOMPOSITION (APTLD) 

分 类 号：O657.3[理学—分析化学]