18β-甘草次酸治疗蛛网膜下腔出血后脑血管痉挛的实验研究  

Effects of 18β-glycyrrhetinic-acid on cerebral vasospasm following experimental subarachnoid hemorrhage in rats

作　　者：何学君 王业忠[1] 刘祺[1] 姬云翔[1] 朱立仓[1] 戴晶[1] 许晖[1] 蔡云鹏[1] 杨传豪[1] 马克涛[1] 赵冬[1] 

机构地区：[1]新疆石河子石河子大学医学院第一附属医院神经外科,832000

出　　处：《中华行为医学与脑科学杂志》2014年第3期243-247,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：新疆生产兵团博士基金资助项目（2011BB016）;石河子大学一般资助项目（ZRKX2010YB23）

摘　　要：目的 探讨缝隙连接阻断剂18β-甘草次酸对蛛网膜下腔出血后脑血管痉挛的治疗作用.方法 120只SD大鼠随机数字表法分对照组、假手术组、手术组及治疗组各30只,且各组分第1,3,5,7,14天5亚组,各亚组6只.视交叉前池二次注血法建立Sprague Dawley大鼠蛛网膜下腔出血动物模型,HE染色鉴定模型,大鼠神经行为学评分、Western blot检测脑血管Cx43表达及全细胞膜片钳技术观察缝隙连接通道电导率变化.结果 神经行为学评分显示,手术组第3,5,7,14天明显下降,分别为（8.33±0.38）分,（6.67±0.20）分,（4.83±0.31）分,（9.33±0.51）分,与对照组、假手术组比较差异有统计学意义（P＜0.01）,不同时间点间比较差异有统计学意义（P＜0.01）,治疗组第3,5,7,14天分别为（10.00±0.65）分,（10.17±0.66）分,（9.83±0.61）分,（10.67±0.70）分,与手术组比较差异有统计学意义（P＜0.01）；Cx43蛋白表达结果显示,手术组第1,3,5,7,14天明显升高,分别为（0.289±0.01）％,（0.389±0.008）％,（0.510±0.006）％,（0.651±0.019）％,（0.329±0.151）％,与对照组、假手术组比较差异有统计学意义（P＜0.01）,不同时间点间比较差异有统计学意义（P＜0.01）,治疗组第1,3,5,7,14天分别为（0.225±0.007）％,（0.228±0.01）％,（0.251±0.02）％,（0.303±0.008）％,（0.267±0.008）％,与手术组比较差异有统计学意义（P＜0.01）；电导率记录结果,手术组第1,3,5,7,14天明显升高,分别为（3.07±0.19） nS/m,（4.88±0.34） nS/m,（8.20±0.31） nS/m,（11.94±0.22） nS/m,（4.07±0.22） nS/m与对照组、假手术组比较差异有统计学意义（P＜0.01）,不同时间点间比较差异有统计学意义（P＜0.01）,治疗组第1,3,5,7,14天分别为（1.38±0.16） nS/m,（1.64±0.15）nS/m,（2.26±0.21） nS/m,（2.89±0.85） nS/m,（2.11±0.20） nS/m,与手术组比较差异有统计学意义（P＜0.01）.结论 缝隙连接阻断剂18β-甘草�Objective To investigate the role of gap junction in the pathomechanisin and treatment of cer- ebral vasospasm after experimental subarachnoid hemorrhage. Methods 120 Sprague Dawley rats were assigned randomly to four groups ： control（ n= 30） , sham group （ n= 30） , operation group （ n= 30） and treatment group （ n= 30） .Animals were killed on the Ist ,3rd ,5th,Tth and 14th day（n=6） respectively.The Sprague Dawley rat two- subarachnoid hemorrhage model was established by injection autogenous femoral artery blood into the front pool of optic chiasma. Neurologic deficit score were tested with Kaoutzanis M scale. HE dye was used to identify the mod- el. The Cx43 proteins in the basilar arteries were detected by Western blot. Whole cell patch clamp technique was used to record the eleetrophysiological changes of gap junction channels in basilar arteries. Results The scores of the neurobehavior significantly decreased in the 3 d ,5 d,7 d and 14 d in operation group（ 8.33±0.38,6.67±0.20, 4.83±0.31,9.33±0.51, respectively） and showed significant difference compared with the control and sham group （P〈0.01） .There was statistically significance between the different time points（P〈0.01 ）.The scores of treatment group were 10.00±0.65,10.17±0.66,9.83±0.61,10.67±0.70 respectively and the difference was statistical signifi- cance（P〈0.01 ） compared with the operation group. The expression of the Cx43 protein increased in the 1 d,3 d,5 d,7 d and 14 d after operation which were （0.289±0.01）%, （0.389±0.008）%, （0.51±0.006）%, （0.651± 0.019） %, （ 0.329±0.151 ） %, respectively and had statistical significance （P〈0.01） compared with the control and sham group. There were statistical significance among the different time points （P〈 0.01 ）.The expression were （ 0.225 ±0.007 ） %, （ 0.228±0.01 ） %, （ 0.251 ±0.02 ） %, （ 0.303±0.008 ） %, （ 0.267 ±0.008 ） % in the I st, 3rd, 5th, 7th,andl4th day alter treatment and had statistical

关 键 词：脑血管痉挛 CX43 18Β-甘草次酸 全细胞膜片钳技术 

分 类 号：R743.35[医药卫生—神经病学与精神病学]