siRNA抑制CDH13表达对膀胱癌5637细胞增殖和侵袭的影响  

The influence of down-regulation of CDH13 expression by siRNA on the proliferation and invasion of bladder cancer 5637 cell

作　　者：林英立[1] 管同郁[1] 戚景光[1] 梁杰[1] 孙光[1] 

机构地区：[1]徐州市肿瘤医院泌尿外科,江苏221005

出　　处：《国际泌尿系统杂志》2014年第2期147-150,共4页International Journal of Urology and Nephrology

摘　　要：目的 探讨通过siRNA抑制CDH13表达对膀胱癌5637细胞增殖和侵袭的影响.方法 以膀胱癌5637细胞为研究对象,用siRNA抑制CDH13表达,用western blot检测干扰后CDH13的表达水平,用MTT法检测细胞增殖,Transwell法检测细胞侵袭能力.结果 western blot检测结果显示干扰组CDH13表达显著减少;干扰组5637细胞增殖明显加快,与空白对照组和阴性对照组相比差异有统计学意义（P＜0.05）;siRNA抑制CDH13表达后细胞的侵袭能力明显增强,与空白对照组和阴性对照组相比差异有统计学意义（P＜0.05）.结论 siRNA能够特异性地抑制CDH13在膀胱癌5637细胞中的表达,CDH13表达减少后细胞增殖加快、侵袭能力增强;CDH13表达减少是膀胱癌发生发展的一个重要促进因素.Objectives The aim of this study was to investigate the influence of down-regulation of CDH13 expression by siRNA on the proliferation and invasion of bladder cancer 5637 cell.Methods Bladder cancer 5637 cells was used to for this study,siRNA was used to down-regulate the CDH13 expression,and then western-blot was used to detect the expression of CDH13,MTT assay was used to determine the proliferation of 5637 cells,the Transwell test was used to detect the invasion ability of the 5637 cells.Results The result of western blot analysis suggested that expression of CDH13 was decreased significantly in interference group.The proliferation ability of 5637 cells in the interference group was accelerated significantly,and the difference was statistically significant compared with the blank control group and negative control group （P 〈 0.05）.The invasion ability of 5637 cells was increased markedly after the inhibition of CDH13 expression by siRNA,and and the difference was statistically significant compared with the blank control group and negative control group（P 〈 0.05）.Conclusions The expression of CDH13 in bladder 5637 cells can be inhibited specifically by siRNA.The proliferation and invasion ability of 5637 cells was accelerated after the down-regulation of CDH13 expression.Down-regulation of CDH13 expression is an important factor to promote the development of bladder cancer.

关 键 词：膀胱肿瘤 细胞增殖 

分 类 号：R737.14[医药卫生—肿瘤]