当归超临界提取物对AOM/DSS诱导的小鼠炎症相关性结直肠癌的化学预防作用  

Chemo-preventive effect of Angelica sinensis' supercritical extracts on AOM /DSS-induced mouse colorectal carcinoma associated with inflammation

作　　者：安静[1] 李晓宁[1] 赵博琛[1] 王琼[1] 兰颐[1] 吴清[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中国中药杂志》2014年第7期1265-1269,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81073059);北京中医药大学创新团队发展计划项目(2011CXTD 13);北京中医药大学校级自主选题(2013-JYBZZ-XS-104)

摘　　要：采用氧化偶氮甲烷/葡聚糖硫酸钠(AOM/DSS)诱导的雄性小鼠结肠癌模型,研究当归超临界提取物对诱发小鼠结肠癌的化学预防作用,并探讨其可能的作用机制。给予雄性Balb/c小鼠单剂量皮下注射AOM(10 mg·kg-1),1周以后,给予2%DSS饮用7 d,诱导结直肠癌。分别按照15,30,60 mg·kg-1灌胃给予药物组当归超临界提取物至第17周。当归超临界提取物组肿瘤发生率、小鼠的荷瘤数及荷瘤体积均低于AOM/DSS模型对照组。这可能与当归超临界提取物能够显著降低AOM/DSS诱导的小鼠炎症相关结直肠癌模型中PCNA,COX-2,iNOS的表达有关。研究结果表明,当归超临界提取物对AOM/DSS诱导的小鼠炎症相关性结直肠癌的发生、发展有一定的干预作用,可进一步用于人类结直肠癌的化学预防研究。To study the chemo-preventive effect of the supercritical extracts from Angelica sinensis （SFE-AS） on induced colorectal carcinoma in mice by using the AOM/DSS-induced male mice colorectal carcinoma model, and discuss its possible action mechanism. Male Balb/c mice were subcutaneously injected with single dose of azoxymethane （AOM,10 mg·kg-1 body weight）. One week later, they were given 2% dextran sodium sulfate （DSS） in drinking water for 7 days to induce colorectal carcinoma. Each drug group was orally administered with supercritical extracts from Angelica sinensis at 15, 30, 60 mg·kg-1 until the 17th week. The tumor incidence rate of the SFE-AS group, mice tumor-bearing quantity and tumor-bearing volume of the SFE-AS group were lower than that of the AOM/DSS model control group, which may be related with the significant reduction of PCNA, COX-2, iNOS in the AOM/DSS-induced mouse colorectal carcinoma model associated with inflammation by SFE-AS. According to the results of this study, SFE-AS showed an intervention effect in the incidence and development of AOM/DSS-induced mouse colorectal carcinoma associated with inflammation, and could be further used in chemo-preventive studies on human colorectal carcinoma.

关 键 词：当归超临界提取物 结直肠癌 氧化偶氮甲烷 葡聚糖硫酸钠 

分 类 号：R735.3[医药卫生—肿瘤]