Monothiol Glutaredoxin-BolA Interactions： Redox Control of Arabidopsis thaliana BolA2 and SufE1  

作　　者：Jeremy Couturier Hui-Chen Wu Tiphaine Dhalleine Henri Pegeot Damien Sudre Jose M. Gualberto Jean-Pierre Jacquot Frederic Gaymard Florence Vignols Nicolas Rouhier 

机构地区：[1]Universite de Lorraine, Interactions Arbres-Microorganismes, UMR1136, F-54500 Vandoeuvre-les-Nancy, France [2]INRA, Interactions Arbres-Microorganismes, UMR1136, F-54280 Champenoux, France [3]CNRS & UMR186 Resistance des Plantes aux Bio-agresseurs, Institut de Recherche pour le Developpement BP 64501, 34394 Montpellier cedex 5, France [4]Biochimie et Physiologie MolEculaire des Plantes, CNRS/INRA/Universite Montpellier 2, SupAgro. Bat 7, 2 place Viala, 34060 Montpellier cedex 1, France [5]Institut de Biologie Moleculaire des Plantes, CNRS UPR2357, F-67084 Strasbourg, France

出　　处：《Molecular Plant》2014年第1期187-205,共19页分子植物（英文版）

摘　　要：A functional relationship between monothiol glutaredoxins and BolAs has been unraveled by genomic analyses and in several high-throughput studies. Phylogenetic analyses coupled to transient expression of green fluo- rescent protein （GFP） fusions indicated that, in addition to the sulfurtransferase SufE1, which contains a C-terminal BolA domain, three BolA isoforms exist in Arabidopsis thaliana, BolA1 being plastidial, BolA2 nucleo-cytoplasmic, and BolA4 dual-targeted to mitochondria and plastids. Binary yeast two-hybrid experiments demonstrated that all BolAs and SufE 1, via its BolA domain, can interact with all monothiol glutaredoxins. Most interactions between protein couples of the same subcellular compartment have been confirmed by bimolecular fluorescence complementation. In vitro experiments indicated that monothiol glutaredoxins could regulate the redox state of BolA2 and SufE1, both proteins possessing a single conserved reactive cysteine. Indeed, a glutathionylated form of SufE1 lost its capacity to activate the cysteine desuifurase, Nfs2, but it is reactivated by plastidial glutaredoxins. Besides, a monomeric glutathionyiated form and a dimeric disulfide-bridged form of BolA2 can be preferentially reduced by the nucleo-cytoplasmic GrxS17. These results indicate that the glutaredoxin-BolA interaction occurs in several subcellular compartments and suggest that a redox regulation mechanism, disconnected from their capacity to form iron-sulfur cluster-bridged heterodimers, may be physiologically relevant for BolA2 and SufE1.A functional relationship between monothiol glutaredoxins and BolAs has been unraveled by genomic analyses and in several high-throughput studies. Phylogenetic analyses coupled to transient expression of green fluo- rescent protein （GFP） fusions indicated that, in addition to the sulfurtransferase SufE1, which contains a C-terminal BolA domain, three BolA isoforms exist in Arabidopsis thaliana, BolA1 being plastidial, BolA2 nucleo-cytoplasmic, and BolA4 dual-targeted to mitochondria and plastids. Binary yeast two-hybrid experiments demonstrated that all BolAs and SufE 1, via its BolA domain, can interact with all monothiol glutaredoxins. Most interactions between protein couples of the same subcellular compartment have been confirmed by bimolecular fluorescence complementation. In vitro experiments indicated that monothiol glutaredoxins could regulate the redox state of BolA2 and SufE1, both proteins possessing a single conserved reactive cysteine. Indeed, a glutathionylated form of SufE1 lost its capacity to activate the cysteine desuifurase, Nfs2, but it is reactivated by plastidial glutaredoxins. Besides, a monomeric glutathionyiated form and a dimeric disulfide-bridged form of BolA2 can be preferentially reduced by the nucleo-cytoplasmic GrxS17. These results indicate that the glutaredoxin-BolA interaction occurs in several subcellular compartments and suggest that a redox regulation mechanism, disconnected from their capacity to form iron-sulfur cluster-bridged heterodimers, may be physiologically relevant for BolA2 and SufE1.

关 键 词：BolA GLUTAREDOXIN INTERACTION PLANTS redox control. 

分 类 号：Q943[生物学—植物学] TQ171.6[化学工程—玻璃工业]