不同钛合金表面形貌对成纤维细胞生物学行为的影响  

The effects of different titanium alloy surface topograph on the biological character of fibroblasts

作　　者：王闻天[1] 孟维艳[1] 周延民[1] 蔡青[1] 王淑艳[2] 王晶晶[1] 刘童斌[1] 

机构地区：[1]吉林大学口腔医院种植科,长春130021 [2]吉林大学口腔医院麻醉科,长春130021

出　　处：《实用口腔医学杂志》2014年第2期151-155,共5页Journal of Practical Stomatology

基　　金：吉林省发展与改革委员会项目(编号:2013C022-6)

摘　　要：目的：比较3种不同钛合金表面形貌对成纤维细胞生物学行为的影响。方法：通过机械加工法（M组）、直流电解蚀刻法（DC 组）、交流电解蚀刻法（AC 组）成形3种不同钛合金表面形貌，制备标准大小试件，使用场发射扫描电子显微镜（FE-SEM）观察其表面微观形态，将 L929成纤维细胞共同培养于钛合金表面，吖啶橙染色法检测初期粘附性；MTT 法检测表面细胞的增殖情况。结果：DC 组钛表面可见直径5～20μm 微孔，AC 组微孔分布更均匀，M组表面可见微沟纹，无微孔。细胞在3组钛片表面粘附良好，AC 组优于 DC 组和 M组。DC 组和 AC 组细胞增殖性优于 M组，第3～5天 AC 组细胞增殖性优于 DC组。结论：通过交流电解蚀刻法形成的兼具微米-亚微米-纳米三级结构的钛合金表面具有良好的生物相容性，能够促进成纤维细胞的早期附着及增殖。Objective：To compare the effect of three different titanium alloy surface topographies on the biological character fibro-blasts.Methods：Three different titanium alloy Ti6Al4V surface topographies were created by machining（M）,direct current electro-chemical etching（DC）and alternating current electrochemical etching（AC）respectively,and standard sized samples were prepared. The surface topographies were observed field emission scanning electron microscopy（FE-SEM）.Mouse L929 fibroblasts were cultured on different surfaces.The attachment of cells was examined by acridine orange staining and observed under fluorescent microscope.The proliferation of cells was measured using MTT assay.Results：Tubles with the diamete of 5 -20 μm were observed on the surface of the sample of group DC,and more evenly distributed tubles on that of group AC.Micro-sulci were observed and no microtuble was found on the sample surface of group M.L929 cells were attached on the surface of all the samples in the 3 groups.More adhered and better strached cells were observed on AC treated surface.The proliferation of fibroblasts on AC and DC surfaces was superior to that on M treated surface and at the 3 -5 day the proliferation of fibroblasts on the AC surface was higher than that on DC treated surface.Con-clusion：AC treated surface with the micro-submicro-nano hierarchical topography of titanium alloy has a superior biocompatibility and can promote the early attachment and proliferation of the fibroblasts.

关 键 词：电解蚀刻 钛合金 软组织 种植体 微米-亚微米-纳米结构 

分 类 号：R783.1[医药卫生—口腔医学]