蔗糖环境影响寡发酵链球菌抑制变形链球菌  

The influence of sucrose concentration environment on the inhibition of Streptococcus oligofermentans on Streptococcus mutans

作　　者：李梦辞 吴菲 杨晓滨[3] 初蕾 刘颖[1] 吴丽更[1] 

机构地区：[1]天津医科大学口腔医院牙体牙髓科,300070 [2]烟台市口腔医院牙体牙髓科 [3]天津大港油田总医院口腔科 [4]烟台市口腔医院儿童牙科

出　　处：《实用口腔医学杂志》2014年第2期156-160,共5页Journal of Practical Stomatology

摘　　要：目的:探讨环境蔗糖对寡发酵链球菌(So)抑制变形链球菌(Sm)作用及对So产过氧化氢能力的影响。方法:用平板培养法观察不同蔗糖环境下So与Sm之间的相互作用;用4-氨基安替吡啉-辣根过氧化物酶法测定So过氧化氢的初始产生速率和产量。结果:500 mmol/L蔗糖环境下未见So对Sm的抑制作用,其它浓度蔗糖环境下So对Sm的抑制作用为:50mmol/L>0 mmol/L和1 mmol/L(P<0.05);不同蔗糖环境下So过氧化氢初始产生速率为:50 mmol/L>0 mmol/L和1 mmol/L>500 mmol/L(P<0.05);过氧化氢总产量为:0 mmol/L和1 mmol/L>50 mmol/L>500 mmol/L(P<0.05)。当So先接种时对Sm的抑制能力优于同时接种。结论:蔗糖浓度影响So对Sm的抑制能力及So产过氧化氢的能力。Objective：To investigate the inhibition of Streptococcus oligofermentans（So）on Streptococcus mutans（Sm）and the hydro-gen peroxide（H2 O2 ）producibility by So under different sucrose concentration environment.Methods：The inhibition of So on Sm was observed by plating method under different sucrose concentration environment.The initial synthesis rates and production of H2 O2 by So were determined by 4-aminoantipyine-horseradish peroxidase method.Results：Under 500 mmol/L of H2 O2 ,the inhibition of So on Sm was not observed.Under the other sucrose concentration environment,the inhibition of So on Sm was as following：50 mmol/L 〉0 mmol/L and 1 mmol/L（P 〈0.05）;the initial synthesis rates of H2 O2 by So under different sucrose concentrations were as following：50 mmol/L〉0 mmol/L and 1 mmol/L〉500 mmol/L（P 〈0.05）;the total production of H2 O2 by So：0 mmol/L and 1 mmol/L 〉50 mmol/L 〉500 mmol/L（P 〈0.05）.When So was inoculated before Sm,the inhibition of So on Sm was stronger than that when the two species were inoculated at the same time.Conclusion：The capability of the inhibition of So on Sm and the production of H2 O2 by So are influenced by sucrose concentration.

关 键 词：寡发酵链球菌 变形链球菌 蔗糖 过氧化氢 

分 类 号：R780.2[医药卫生—口腔医学]