烟草浸提液对人牙周膜细胞HSP70表达的影响  

Effects of tobacco extract on the proliferation and HSP70 expression of human periodontal ligament cells

作　　者：李玮[1,2] 陈栋[1] 李坤阳[1] 范云[1] 余方方[1] 

机构地区：[1]郑州大学口腔医学院,450052 [2]郑州人民医院颐和医院口腔科

出　　处：《实用口腔医学杂志》2014年第2期211-214,共4页Journal of Practical Stomatology

基　　金：郑州市科技局科技攻关项目(编号:112PPTSF299-2)

摘　　要：目的：观察烟草浸提液（ST）对人牙周膜细胞（hPDLCs）增殖、热休克蛋白70（HSP70）表达的影响。方法：常规培养hPDLCs，第5～8代细胞用于实验。对照组为常规培养液，实验组为含不同浓度的 ST 培养液（0．016～50 g/L ST），作用24 h后 MTT 法检测细胞增殖、免疫组化和 Western Blot 法检测细胞 HSP70的表达。结果：ST 浓度为0．4～50 g/L 时抑制 hPDLCs增殖，增强细胞质及胞核内 HSP70的表达；ST 浓度增加，其作用更明显。结论：ST 可抑制 hPDLCs 增殖，促进 HSP70呈表达。Objective：To study the effects of smokeless tobacco extract（ST）on the proliferation and the heat shock protein 70 （HSP70）expression of human periodontal ligament cells（hPDLCs）.Methods：hPDLCs were cultured in vitro and identified by im-munohistochemistry（IHC）.The cells were stimulated with ST at 0.01 6 -50 g/L respectively for 24 h,the proliferation was examine by MTT assay,HSP70 expression was detected by immunehistochemical staining and Western Blot.Results：ST inhibited the prolifer-ation and increased HSP70 expression in cytoplasm and nucleus at 0.4 -50 g/L dose dependantly.Conclusion：ST may inhibit the proliferation and increase HSP70 expression of hPDLCs in a dose depandant manner.

关 键 词：牙周膜细胞(hPDLCs) 烟草 HSP70 

分 类 号：R781.42[医药卫生—口腔医学]