青海湖地区5株H9N2亚型禽流感病毒全基因组序列进化分析  

Complete Genome Phylogenetic Analysis of Five H9N2 Avian Influenza Viruses Isolated from Poultry Flocks in Qinghai Lake Region

作　　者：刘琳玉 姜双应 汪立杰[1] 易虎[2] 赵生仓 唐志坚[2] 徐翠玲[1] 董婕[1] 高荣保[1] 张烨[1] 邹淑梅[1] 李晓丹[1] 杨磊[1] 杨静[1] 陈涛[1] 舒跃龙[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所国家流感中心世界卫生组织流感参比和研究合作中心,北京102206 [2]青海省疾病预防控制中心,西宁810007

出　　处：《病毒学报》2014年第2期109-118,共10页Chinese Journal of Virology

基　　金：WHO 2012/2013年度加强西部省份人禽流感监测能力项目;国家重点基础研究发展973计划课题(2011CB504704);国家自然科学基金(81373107)

摘　　要：2012年7～9月从来源于青海湖地区活禽市场的环境样本中分离到5株H9N2亚型禽流感病毒，为了了解其基因遗传进化情况，本研究通过RT—PCR技术扩增分离毒株的8个基因片段，并进行全基因序列测定。对其分子特征及全基因序列进行遗传进化分析。结果显示5株病毒的HA基因片段的核苷酸相似度为93．2％～99．1％。NA基因核苷酸的相似度为94．5％～99．8％。A／environment／qinghai／017／2012的裂解位点为PSKSSRGLF，其它4个毒株的HA裂解位点均为PSRSSRGLF。5个病毒的HA基因第226位受体结合位点均为L。M1基因片段中发生了N30D和T215A替换。遗传进化分析表明5株病毒同2005年湖南分离的A／chicken／Hunan／5260／2005（HgN2）毒株类似，为一种重配基因型禽流感病毒。其中HA、NA、NS基因片段属于Y280-like支系，MP基因片段属于G1-like支系，NP、PBl、PB2、PA四个基因片段属于F98-like支系。Five H9N2 avian influenza virus strains were isolated from the environmental samples in live poultry market in Qinghai Lake region from July to September, 2012. To evaluate the phylogenetic charac- teristics of these H9N2 isolates, the eight gene segments were amplified by RT-PCR and sequenced. The phylogenetic and molecular characteristics of the five strains were analyzed. The results showed that the HA genes of five strains shared 93.2%-99.1% nucleotide identities with each other, and the NA genes shared 94.5 %-99.8% nucleotide identities. The HA cleavage site sequence of the A/environment/qinghai/ 017/2012 isolate was PSKSSRGLF, and the HA cleavage site sequences of the other four strains were all PSRSSRGLF. The HA receptor-binding site had the Q226L mutation. The M1 gene segment had the N30D and T215A mutations. The phylogenetic analysis showed that the five strains were similar to the vi- rus A/chieken/Hunan/5260/2005 （H9N2） isolated in Hunan Province, China and were reassortant geno- type viruses; the HA, NA, and NS genes belonged to the Y280-1ike lineage; the MP gene belonged to the Gl-like lineage; the NP, PB1, PB2, and PA genes belonged to the F98-1ike lineage.

关 键 词：H9N2 禽流感病毒 基因组 分子特征 遗传进化分析 

分 类 号：R373.1[医药卫生—病原生物学]