水产品中孔雀石绿和结晶紫及其代谢产物的高效液相色谱测定法  

Determination of the malachite green and crystal violet in aquatic products by HPLC

作　　者：向仲朝[1] 岳蕴瑶[1] 张婷[1] 李佳佳[1] 

机构地区：[1]绵阳市疾病预防控制中心,四川绵阳621000

出　　处：《中国卫生检验杂志》2014年第6期788-790,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立水产品中孔雀石绿、隐色孔雀石绿、结晶紫、隐色结晶紫的高效液相色谱定量分析方法。方法对样品的提取方法,测定条件,方法的线性范围和精密度,准确度等进行实验。结果样品用乙腈直接提取,不加衍生试剂,不采用柱后衍生的方法,选用适宜的色谱柱在不同波长下同时测定样品中孔雀石绿、结晶紫、隐色孔雀石绿、隐色结晶紫的含量。在0μg/ml^10.0μg/ml范围内,浓度与峰面积呈直线相关关系;方法最低检出量为0.00020μg;按本法测定,最低检出浓度为0.0020 mg/kg。对加标量为0.025 mg/kg和0.050 mg/kg的样品,连续测定5次,相对标准偏差RSD为2.8%~9.1%,回收率为72%~96%。结论本法具有样品提取简单、稳定,测定简便、灵敏,结果精密、准确的特点,适用于水产品中孔雀石绿、隐色孔雀石绿、结晶紫、隐色结晶紫的测定。适合无液相色谱-质谱仪的基层单位推广应用。Objective To establish a high performance liquid chromatographic method （HPLC） for determination of malachite green, leueomalaehite green, crystal violet and leueoerystal violet in aquatic products. Methods The sampling method and de- termination condition of the aquatic products, linear range, precision and accuracy of the method were studied. Results Mala- chite green and crystal violet in aquatic products were extractly directly with aeetonitrile. The linear range of the method was 0 μg/ml - 10.0 μg/ml. The limit of detection was 0. 00020 μg, the lowest detection concentration was 0. 0020 mg/kg. The RSDs were in the range of 2.8% -9.1% for samples with concentration from 0.025 mg/kg to 0.050 mg/kg （n =5） , and the recovery rates were 72% - 96%. Conclusion This method is simple, rapid, precise, accurate and applicable for determina- tion of malachite green, leueomalachite green, crystal violet and leueoerystal violet in aquatic products, it is also worthy of pop- ularization in grassroot units without liquid chromatography- mass spectrometer.

关 键 词：孔雀石绿 结晶紫 液相色谱 水产品 

分 类 号：O657.72[理学—分析化学]