氧化葡萄糖酸杆菌的固定化及在鼓泡式反应器中制备乙醇酸  

Immobilization of Gluconobacter oxydans Cells for the Preparation of Glycolic Acid in Bubbling Reactor

作　　者：魏胜华[1,2] 王卫军[1] 孟娜[1,2] 陶玉贵[1,2] 吴丁柱 朱龙宝[1,2] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000 [2]微生物发酵安徽省工程研究中心,安徽芜湖241000

出　　处：《精细化工》2014年第4期442-446,共5页Fine Chemicals

基　　金：安徽工程大学人才引进基金(2007YQ006)~~

摘　　要：考察了包埋法固定氧化葡萄糖酸杆菌的过程,并且研究了利用该固定化细胞在鼓泡塔生物反应器中转化乙二醇制备乙醇酸。固定化的最优条件为:聚乙烯醇的最佳质量分数为8%,海藻酸钠为0.8%,最适湿细胞包埋量为每毫升凝胶0.27 g。固定化细胞对酸碱的抗性和热稳定性均较游离细胞有所提高。利用固定化细胞在鼓泡式反应器中转化乙二醇制备乙醇酸,在乙二醇质量浓度70 g/L的条件下,首批转化率达到92.6%,并且可以实现连续转化6个批次,平均转化率在85%以上。The immobilization of Gluconobacter oxydans cells and the conversion of ethylene glycol into glycolic acid in bubbling reactor were investigated. The optimum conditions for the immobilization were 8% PVA,0. 8% sodium alginate and 0.27 g cells biomass per milliliter gel. The immobilized cells were more stable than free cells in pH and meanwhile with better thermal stability. The conversion of ethylene glycol into glycolic acid was carried out in a bubbling reactor. When the initial ethylene glycol was 70 g/L, the first cycle conversion was 92.6%, and it could successively convert for six cycles, and the average conversion was about 85%.

关 键 词：乙醇酸 乙二醇 氧化葡萄糖酸杆菌 固定化 鼓泡塔反应器 生物工程 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]