利用种子蛋白SDS-PAGE电泳谱带鉴定菜豆品种真实性与纯度的研究  

Study on Trueness and Purity Identification of Seeds in Common Bean(Phaseolus vulgaris L.) by SDS-PAGE Ptotein Electrophoresis Bands

作　　者：张雪[1,2] 李爽[1] 韩玉珠[1] 

机构地区：[1]吉林农业大学园艺学院,吉林长春130118 [2]吉林省农业科学院农村能源研究所,吉林长春130033

出　　处：《北方园艺》2014年第7期91-95,共5页Northern Horticulture

基　　金：长春市科技局资助项目(长科技合(2011230)号)

摘　　要：以不同采收年限的17个菜豆品种的种子为试材,采用SDS-PAGE电泳方法获得菜豆种子贮藏蛋白的电泳图谱,比较分析其真实性;同时研究种子蛋白SDS-PAGE技术鉴定菜豆品种真实性和纯度的可行性。结果表明:17个菜豆品种可以通过SDS-PAGE方法得到特征谱带A、B、C、D、E、F、G、H,且各品种谱带差异明显;SDS-PAGE蛋白质电泳方法用于鉴定菜豆品种真实性和种子纯度是可行的。Taking seeds of seventeen common bean in diffrent recovery years as materials,the seeds storage proteins with SDS-PAGE electrophoresis were analysized. The feasibility of trueness and purity identification of seeds in common bean by SDS-PAGE ptotein electrophoresis bands were studied. The results shouwed that the unique bands were A,B,C,D,E, F,G and H for seventeen common bean, and there were significant difference among species. It was possible that SDS-PAGE could be used to identified the purity and trueness of seventeen landraees of common beans.

关 键 词：菜豆 SDS-PAGE 真实性 纯度 

分 类 号：S643.1[农业科学—蔬菜学]