黄芪甲苷对实验性大鼠重症急性胰腺炎的治疗作用  

Therapeutic effects of astragaloside Ⅳ on severe acute pancreatitis in rats

作　　者：邱雷[1] 史建华[2] 殷国建[1] 胡国勇[3] 王兴鹏[3] 赵严[1] 

机构地区：[1]同济大学附属第十人民医院消化科,上海200072 [2]中国人民解放军第411医院分院 [3]上海交通大学附属第一人民医院消化科

出　　处：《上海医学》2014年第3期227-230,I0003,共5页Shanghai Medical Journal

摘　　要：目的研究黄芪甲苷对大鼠重症急性胰腺炎(SAP)的治疗作用。方法将51只健康雄性Sprague-Dawley大鼠分入正常对照组(9只)、SAP模型组(21只)和黄芪甲苷治疗组(21只)。SAP模型组通过逆行胆胰管注射4%牛黄胆酸钠制作大鼠SAP模型;黄芪甲苷治疗组则在大鼠造模前12和2h分别予腹腔内注射黄芪甲苷(40mg/kg)进行预处理,造模方法与SAP模型组相同。于造模后12、24、48h取材,测定血清淀粉酶、脂肪酶水平,采用酶联免疫吸附试验检测血清白细胞介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α水平,对胰腺组织进行病理学评分并观察造模后的存活率。结果正常对照组和黄芪甲苷治疗组大鼠均无死亡,SAP模型组造模后12、24、48h时分别有1、2、1只大鼠死亡。SAP模型组和黄芪甲苷治疗组造模后12、24、48h时的血清淀粉酶、脂肪酶水平和造模后24h时胰腺组织的病理学评分均较正常对照组同时间点显著升高(P值均<0.05)。黄芪甲苷治疗组造模后12、24、48h时的血清淀粉酶、脂肪酶水平,造模后24h时的IL-1β、IL-6、TNF-α水平和胰腺组织病理学评分均较SAP模型组同时间点显著降低(P值均<0.05)。正常对照组大鼠在造模后12、24、48h时的胰腺组织无明显损伤;SAP模型组大鼠胰腺组织间质明显水肿,叶间隔、小叶间隔和腺泡间隔增宽,腺泡细胞空泡样变形、片状坏死,伴血管充血、血管周围和间质内炎性细胞浸润;黄芪甲苷治疗组大鼠胰腺组织水肿、坏死、出血和炎性细胞浸润均较SAP模型组显著改善。结论黄芪甲苷对SAP大鼠具有治疗作用,其机制可能与能够负性调节促炎因子IL-1β、IL-6、TNF-α的表达有关。Objective To study the therapeutic effects of astragaloside IV on severe acute pancreatitis （SAP） in rats. Methods Fifty-one healthy male Sprague-Dawley rats were enrolled in this study and divided into three groups： blank control group （n = 9）, SAP group （ n = 21 ） and astragaloside pretreatment group （n = 21 ）. SAP was induced by retrograde cholangiopancreatic ductal injection of 4 % sodium taurocholate in the rats of SAP group and astragaloside pretreatment group. Astragaloside IV （40 mg/kg） was intraperitoneally injected 12 h and 2 h before the induction of SAP in the rats of astragaloside pretreatment group. The rats were sacrificed 12 h, 24 h and 48 h after SAP induction. Serum amylase, lipase, interleukin（IL）-1β, IL-6 and tumor necrosis factor （TNF）-a levels were measured by biochemistry and enzyme linked immunosorbent assay （ELISA）. The pathological assessment of pancreatic tissue was conduct and survival rate of rats was calculated after SAP induction. Results No rats died in the blank control group and astragaloside pretreatment group, while 4 rats died in the SAP group （one at 12 h after SAP induction, two at 24 h, and another one at 48 h）. Compared with those in the blank control group, the levels of serum amylase and lipase at 12 h, 24 h and 48 h after SAP induction and the pathological score of pancreatic tissue at 24 h after SAP induction were significantly increased in both SAP group and astragaloside pretreatment groups （all P〈0.05）. The levels of serum amylase and lipase at 12 h, 24 h and 48 h after SAP induction and the levels of IL-1β, IL-6 and TNF-a and pathological score of pancreatic tissue at 24 h after SAP induction were significantly higher in astragaloside pretreatment group than those in SAP group （all P〈0.05）. In blank control group, we did not find obvious inflammatory injury of pancreatic tissue. In SAP group, edema appeared in pancreatic interstitial tissue, interlobular septum and gland alveolus septum were widened, deformation

关 键 词：黄芪甲苷 重症急性胰腺炎 坏死 

分 类 号：R576[医药卫生—消化系统]