杭白菊辐照灭菌效果及对功效成分和指纹图谱影响  

Irraition Effect on Sterilization and the Function Component and Fingerprint of Flos Chrysanthemi

作　　者：黄卫平[1] 吴小平[2] 唐靓[2] 刘放[2] 

机构地区：[1]浙江医学高等专科学校,浙江杭州310053 [2]浙江省医学科学院药物研究所,浙江杭州310013

出　　处：《中华中医药学刊》2014年第4期835-838,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科研基金项目(2009CB0028)

摘　　要：目的：研究了用^60Co-γ射线辐照杭白菊杀茵效果及对功能成份和指纹图谱的影响。方法：采用高效液相色谱法进行定量分析和指纹图谱研究，并进行卫生学检查。结果：结果表明，杀菌效果随着辐照剂量的增加而显著增加，杂茵和霉菌的D10值分别为3．71key和3．75kCy。绿原酸含量和指纹图谱相似度随辐照剂量的增加而下降。结论：根据杀茵效果和功能成分因素综合考虑，认为杭白菊的辐照杀菌有效剂量以3．0～5．0kCy为宜。辐照灭茵后的杭白菊有较好的耐贮藏性，保质期延长。Objective ： The E effect of ^60Co-γ ray irradiation on sterilization and the function components and finger- print in of Flos Chrysanthemi was investigated. Methods ： The content determination of chlorogenic acid in Flos Chrysanthe- mi and fingerprint by HPLC and health were checktested. Results ： It showed that sterilization effect increases increased sig- nificantly with the increasing irradiation dose. The D~ value forbaeteria and moulds were 3.71 kGy and 3.75 kGy, respec- tively. The contents of ehlomgenic acid and fingerprint similarity decreased with the increase of irradiation dose. Conclu- sion： Based on the data of sterilization and product quality,3 - 5 kGy irradiation is recommended for Flos Chrysanthemisterilization. The Flos Chrysanthemi after irradiative sterilization has a better storage property, and thus enhancing the quality guarantee period of the fermented powder.

关 键 词：辐照杀菌 杭白菊 功能成分 指纹图谱 

分 类 号：R284.3[医药卫生—中药学]