颅痛定对人脑缺血-再灌注患者血清 NO及 CO 浓度的影响  

Effects of L-tetrahydropalmatine on nitric oxide and carbon monoxide concentration in plasma during cerebral ischemia reperfusion

作　　者：何彩霞[1] 陈齐国[1] 卜珊珊[1] 何浩[1] 吴华[1] 文体端[1] 李沅湘[1] 

机构地区：[1]长沙市第四医院重症医学科, 湖南 长沙410006

出　　处：《中国急救医学》2014年第4期318-322,共5页Chinese Journal of Critical Care Medicine

基　　金：湖南省卫生厅资助项目（湘卫科教发[2007]7号）

摘　　要：目的：观察脑缺血-再灌注对血清NO和CO的影响及颅痛定的干预。方法随机抽样2011-01～2012-12收治的心肺复苏术后35例患者，随机分为治疗组20例，对照组15例。两组患者心肺复苏后立即给予一般性治疗，治疗组起病后即加用颅痛定60 mg，每日3次，持续3 d用药。于心肺复苏术后6、24、48、72 h抽血测定NO、CO浓度。硝酸还原酶法测定血清NO浓度，可见分光光度计法测定CO浓度。结果人脑缺血-再灌注6、24、48、72 h后外周静脉血中NO浓度增加，其中脑缺血＜5 min再灌注48 h达到高峰；人脑缺血-再灌注6 h后CO浓度最大，之后回落，其中脑缺血＜5 min再灌注24 h达到高峰；颅痛定降低脑缺血-再灌注6～24 h CO浓度，增加NO浓度，而在缺血-再灌注24～72 h降低NO浓度。结论颅痛定可能通过调控机体内NO和CO浓度，发挥抗脑缺血-再灌注损伤作用。Objective To observe the effects of L -tetrahydropalmatine（L -THP）on nitric oxide （NO） and carbon monoxide （CO） concentration in plasma during cerebral ischemia reperfusion . Methods Cardiopulmonary resuscitation （ CPR） of 35 cases of postoperative patients were divided into 2 groups in random：treatment group（n=20）and control group（n=15）.Two groups of patients after CPR immediately to give general treatment .Treatment group after the onset of the cranial pain .Nitrate reductive enzymatic method was used to detect the serum content of NO; visible spectrophotometer method was used to detect the serum content of CO .Results NO content was elevated at 6, 24, 48, 72 hour, and peaked at 48 hour when cerebral ischemia occurred within 5 minutes;CO content peaked at 6 hour, while peaked at 24 hour when cerebral ischemia occurred within 5 minutes;L-THP significantly increased NO concentrations and reduced CO concentrations after cerebral ischemia reperfusion from 6 to 24 hour, while reduced NO concentrations after cerebral ischemia reperfusion from 24 to 72 hour. Conclusion L-THP probably has a good effect on cerebral ischemia -reperfusion injury by regulating the NO and CO contents .

关 键 词：颅痛定 一氧化氮( NO) 一氧化碳( CO) 脑缺血-再灌注 

分 类 号：R743.31[医药卫生—神经病学与精神病学]