肿瘤抑素T42肽对肝癌移植瘤生长的抑制作用  

Inhibitory effect of tumstatin T42 pcptide on growth of implanted hepatocellular carcinoma

作　　者：郑幼伟[1] 刘海潮[1] 买二辉[1] 孟冬冬[1] 苏宝威[1] 董瑞强[1] 

机构地区：[1]郑州大学附属洛阳中心医院肝胆疝外科,洛阳471001

出　　处：《中华实验外科杂志》2014年第4期763-764,I0001,共3页Chinese Journal of Experimental Surgery

基　　金：洛阳市科研资助项目（1101049A）

摘　　要：目的探讨肿瘤抑素T42肽在人肝癌裸鼠种植瘤模型诱导凋亡中的作用及机制。方法建立人肝癌细胞株HCCLM9裸鼠模型，治疗组和对照组（n=10）分别皮下注射T42肽和生理盐水；治疗2周后收集标本，采用原位缺口末端标记法（TUNEL）检测细胞凋亡水平，免疫组织化学方法检测半胱氨酰天冬氨酸特异性蛋白酶（Caspase）-3蛋白的表达。结果T42肽治疗组肝癌细胞凋亡指数为（8．48±O．58）％，对照组为（2．564±0．48）％，T42肽治疗组显著高于对照组（P〈0．05）；Caspase-3蛋白在实验组中的阳性表达率为80％，在对照组的阳性表达率为30％，T42肽组显著高于对照组（P〈0．05）；治疗后T42肽治疗组的瘤体重量为（1．83±0．22）g，对照组为（2．11±1．67）g，T42肽治疗组显著低于对照组（P〈0．05）。结论T42肽在人肝癌裸鼠模型中可诱导肝癌细胞凋亡，Caspase-3的表达可能在T42肽诱导细胞凋亡的过程中有重要作用。Objective To study the effect of tumstatin T42 peptide on the induced apoptosis of hu man hepatocellular carcinoma xenografts in nude mice and the possible mechanism. Methods The human hepatoma cell line HCCLM9 nude model was established. The treatment group and control group （ n = 10 each） were injected subcutaneously with T42 peptide and saline respectively. The specimens after treat- ment for two weeks were collected. TdT-mediated dUTP nick end labeling （TUNEL） method was used to detect apoptosis levels, and immunohistoehemistry to detect Caspase-3 protein expression. Results The apoptosis index in the T42 peptide treatment group was （ 8.48 ± 0. 58 ） % , and that in the control group was （2. 56 ±0.48） % （P 〈 0. 05）. The expression of Caspase-3 in the T42 treatment group was signifi cantly higher than that in the control group （80% vs. 30% ,P 〈 0. 05）. After treatment, the tumor weight in the T42 treatment group was （ 1.83 ± 0. 22 ） g and that in the control group was （2. 11 ± 1.67 ） g （ P 〈 0. 05 ）. Conclusion T42 peptide can induce apoptosis of hepatoma cells in human hepatocellular carcino ma of nude mice, which may be contributed to the expression of Casnase-3.

关 键 词：癌 肝细胞 肿瘤抑素 T42肽 半胱氨酰天冬氨酸特异性蛋白酶-3 

分 类 号：R735.7[医药卫生—肿瘤]