三磷酸腺苷结合转运家族蛋白4在胆管癌中的表达及作用  

Expression and effect of adenosine triphosphate-binding cassette transporter sub-family class 4 incholangiocarcinoma

作　　者：张文杰[1] 王雪峰[1] 顾钧[1] 陶怡菁[1] 陈磊[1] 吕文杰[1] 毕建威[2] 

机构地区：[1]上海交通大学医学院附属新华医院普外科,200092 [2]第二军医大学附属长海医院普外科

出　　处：《中华实验外科杂志》2014年第4期853-855,共3页Chinese Journal of Experimental Surgery

基　　金：上海市科委基金资助项目（122R1420100）

摘　　要：目的观察i磷酸腺苷结合转运家族蛋白4（ABCC4）在胆管癌细胞增殖和转移中的作用。方法收集2010年6月到2012年6月病理检查确诊胆管癌39例，实时定量聚合酶链反应（Real-timePCR）检测人胆管癌与正常胆管黏膜标本中ABCC4的含量；在人胆管癌细胞株中沉默ABCC4基因；噻唑蓝（MTT）法绘制生长fIff线；克隆培养后进行统计。结果人胆管癌细胞中ABCC4异常高表达，Western blot实验验证在胆管癌细胞中沉默ABCC4基因表达成功，ABCC4基因沉默的HuCCTl细胞增殖速率较对照组明显减缓（P〈0．05），克隆实验显示下调ABCC4蛋白表达能够明显降低胆管癌细胞的恶性表征。结论ABCC4在胆管癌细胞中具有癌基因的功能。Objective To investigate the effect of adenosine triphosphate （ATP）-binding cassette transporter sub-family class 4 （ABCC4） on proliferation and metastasis of cholangiocareinoma cells. Meth ods There were 39 cases of cholangiocarcinomas from June 2010 to June 2012. Real-time quantitative polymerase chain reaction （ Real-time PCR） was used to detect the ABCC4 expression in human cholangio- carcinoma and normal bile duct mucosa. Then ABCC4 gene was silenced in one human eholangiocareinoma cell line. Growth curve was drawn by using methyl thiazol tetrazolium （MTF） assay. Results The human eholangiocarcinoma cells had a abnormally higher expression of ABCC4. The silencing of ABCC4 was con firmed in cholangiocarcinoma cells by Western blotting. ABCC4 gene-sileneed HuCCT1 ceils proliferated significantly slower than the control group （ P 〈 0. 05 ）. Clone experiments showed that the lowered ABCC4 protein could significantly reduce the malignant phenotypes. Conclusion We speculate that ABCC4 in eholangiocarcinoma cells functions as the role of oncogenes.

关 键 词：三磷酸腺苷结合转运家族蛋白4 RNA干扰 胆管癌 

分 类 号：R735.8[医药卫生—肿瘤]