紧密连接蛋白1和血管内皮生长因子C在大肠癌表达的相关性  

Correlation of Ciaudin-1 and vascular endothelial growth factor C expeession in colorectai cancer

作　　者：张国建[1] 阮洪训 阎庆辉[1] 王凤安[1] 吴爱须[1] 秦毅[1] 

机构地区：[1]河北医科大学第二医院外科,石家庄050000

出　　处：《中华实验外科杂志》2014年第4期859-861,I0002,F0003,共5页Chinese Journal of Experimental Surgery

摘　　要：目的探讨紧密连接蛋白1（Claudin-1）和血管内皮生长因子C（VEGF-C）蛋白在大肠癌发生发展中的相关性。方法应用链霉菌抗生物素蛋白-过氧化物酶（sP）法检测45例大肠癌组织、癌旁组织以及20例大肠组织中2个基因的表达，并进行相关分析。结果大肠癌组织中的C｜audin-l表达阳性率明显高于癌旁组织和正常组织（60．0％、33．3％和10．0％），VEGF—C表达阳性率明显高于癌旁组织和正常组织（64．4％、26．7％和10．0％），并且两者的表达均与肿瘤分化程度、浸润深度、淋巴结转移以及临床分期密切相关，两者的表达呈直线正相关。结论Claudin-1可能通过VEGF-C信号途径参与大肠癌的发生发展。Objective To investigate the correlation of Claudin-1 and vascular endothelial growth factor C （ VEGF-C ） protein expression in colorectal cancer development. Methods The immunohisto chemical staining [ streptavid-in-peroxidase （SP） method] was done to detect the expression of Claudin-1 and VEGF-C in 45 cases of colorectal cancer tissues and the corresponding tissues adjacent to carcinoma, and 20 cases of normal colon mucosa tissues. Results The positive expression rate of Claudin-1 in cancer tissues was significantly higher than that in the tissues adjacent to carcinoma and normal tissues （60. 0%, 33.3% and 10.0% ）. The positive expression rate of VEGF-C in cancer tissues was significantly higher than that in the tissues adjacent to carcinoma and normal tissues （64. 4% , 26. 7% and 10. 0% ）. Both Claudin-1 and VEGF-C expression was correlated with tumor differentiation, depth of invasion, lymph node metastasis and TNM stage. The expression of Claudin-1 and VEGF-C had a linear correlation. Conclusion Claudin-1 is involved in the development of colorectal cancer possibly through VEGF-C signaling pathways.

关 键 词：紧密连接蛋白1 血管内皮生长因子C 大肠癌 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]