BTG2作为一种新的电离辐射诱导基因的研究  

Study on B-cell translocation gene 2 as a novel radiation-induced gene

作　　者：李敏[1] 贺欣[2] 周则卫[2] 孟庆慧[1] 樊赛军[1,2] 

机构地区：[1]苏州大学医学部放射医学与防护学院肿瘤放射生物学实验室,215123 [2]中国医学科学院放射医学研究所,天津市放射医学与分子核医学重点实验室,天津300192

出　　处：《国际放射医学核医学杂志》2014年第2期71-74,共4页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：国家自然科学基金（81071906,81172127）

摘　　要：目的 研究γ射线照射对肿瘤细胞的B细胞易位基因2（BTG2）表达水平的影响.方法 应用RT-PCR方法研究电离辐射对肿瘤细胞BTG2 mRNA水平的影响;应用Western blot方法测定电离辐射对肿瘤细胞BTG2蛋白表达水平的影响.结果 与未照射对照细胞相比,3 Gyγ射线单次剂量照射明显提高了人乳腺癌细胞系MCF-7、MDA-MB-231和人宫颈癌细胞系C33A、HeLa中的BTG2蛋白的表达水平.γ射线照射人乳腺癌细胞系MCF-7和MDA-MB-231导致了照射剂量和时间依赖性的BTG2表达水平的升高,而且这种改变既表现在BTG2 mRNA水平上,也反映在BTG2蛋白水平上.结论 BTG2是一种新的辐射诱导DNA损伤的反应基因,进一步研究将为BTG2作为肿瘤放射治疗的疗效和预后评价指标提供实验基础和依据.Objective To investigate the effects of ionizing radiation on the expression of B-cell translocation gene 2 （BTG2） in cancer cells.Methods RT-PCR was used to determine the level of BTG2 mRNA,while the expression of BTG2 protein was assayed by Western blot.Results Compared with the unirradiated cells,a single dose of 3 Gy γ-rays caused a significant increase of BTG2 protein in breast cancer cell lines,MCF-7 and MDA-MB-231,and cervical cancer cell lines,C33A and HeLa.Furthermore,irradiation with γ-rays resulted in a dose and time dependent increase of BTG2 at both mRNA and protein levels.Conclusion The present study demonstrates,for the first time,that BTG2 is a value response gene to exposure of ionizing radiation,which may provide evidence for BTG2 as a novel marker for radiotherapy of cancer.

关 键 词：肿瘤 辐射 电离 B细胞易位基因2 

分 类 号：R730.5[医药卫生—肿瘤]