高浓度脂多糖对骨唾液酸蛋白和IL-8基因表达的影响  

Effect of high concentration of Lipopolysaccharide on the Bone Sialoprotein and IL-8 gene expression

作　　者：张研[1] 李新月[1] 

机构地区：[1]天津市口腔医院,300041

出　　处：《现代口腔医学杂志》2014年第2期107-110,共4页Journal of Modern Stomatology

基　　金：天津市卫生局科技基金(09KY20)

摘　　要：目的研究高浓度牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g.)脂多糖(lipopolysaccharide,LPS)对人牙周膜成纤维细胞(human periodontal ligament fibroblasts,hPDLFs)中骨唾液酸蛋白(Bone sialoprotein,BSP)和白细胞介素8(Interleukin 8,IL-8)基因表达的调节。方法 10mg/L P.g.LPS处理第4-5代体外培养的hPDLFs不同时间(1、4、8、12、24小时)后,采用实时荧光定量PCR(Real-time PCR)检测骨唾液酸蛋白(BSP)和IL-8基因的表达水平。结果 10mg/L P.g.LPS作用hPDLFs 1、4小时后BSP mRNA的表达水平稍有下降(P>0.05),并在刺激8、12、24小时后BSP mRNA的表达水平显著下降(P<0.01);此外10mg/L P.g.LPS明显上调hPDLFs中IL-8 mRNA表达水平,在处理8h时刺激作用最为明显,达到高峰(P<0.01)。结论高浓度(10mg/L)P.g.LPS对hPDLFs的BSP基因表达有抑制作用,而对IL-8基因表达有增强作用。Objective To investigate the regulation of gene expression of bone sialoprotein （BSP） hand Interleukin 8 （IL-8） by high concentration of Porphyromonas gingivalis lipopolysaecharide （P.g.LPS） in human periodontal ligament fibroblasts （hPDLFs）. Methods hPDLFs cells were cultured in 10mg/L P.g. LPS for time course （1,4,8,12,24h）, total RNA were extracted, and then BSPand IL-8 mRNA levels were observed by Real-time PCR. Results l0mg/L P.g. LPS decreased the BSP mRNA expression in hPDLFs after stimulation for 8h （P〈0.01）, while IL- 8 mRNA levels were up-regulated by 10mg/L P.g. LPS at 8h （P〈0.01）. Conclusion The effects of high concentration of P.g. LPS （l0mg/L） on the BSP mRNA levels in hPDLFs were suppressed, whereas on the IL-8 mRNA expression were enhanced.

关 键 词：牙龈卟啉单胞菌 脂多糖 牙周膜成纤维细胞 骨唾液酸蛋白 白细胞介素-8 

分 类 号：R780.2[医药卫生—口腔医学]