丙酮酸盐对阿尔茨海默病大鼠学习记忆的影响  

Effects of pyruvate on learning and memory in a rat model of Alzheimer's disease

作　　者：王晓楠[1] 胡雪君[1] 杨旸[1] 王斯琪[1] 

机构地区：[1]中国医科大学附属第一医院呼吸感染科,沈阳110001

出　　处：《中国神经精神疾病杂志》2014年第2期87-90,共4页Chinese Journal of Nervous and Mental Diseases

基　　金：国家自然科学基金资助项目(编号:81200246);高等学校博士学科点专项科研基金(编号:20122104120004);辽宁省自然科学基金项目(编号:2013010025-401)

摘　　要：目的研究丙酮酸盐对阿尔茨海默病(Alzheimer’s disease,AD)模型大鼠学习记忆的影响并探讨其与海马神经细胞死亡的关系。方法将36只wistar大鼠随机分为空白对照组、AD模型组和丙酮酸盐治疗组,根据实验要求给药处理6周,同时观察各组大鼠一般情况,Morris水迷宫实验检测大鼠学习记忆能力,苏木精-伊红(hematoxylin and eosin,HE)染色检测海马神经细胞死亡,利用2′,7′-dichlorodihydrofluorescein diacetate(H2DCF-DA)来检测海马CA1区内活性氧(reactive oxygen species,ROS)聚集情况。结果丙酮酸盐治疗组与AD模型组比较,一般情况均明显改善,逃避潜伏期均明显缩短(41.37±6.24,31.67±7.26,24.26±7.16,20.45±5.72,19.36±5.89,均P<0.05);原平台象限活动时间明显增加(50.36±7.46 s,P<0.05),穿越原平台次数明显增多(3.98±1.23次/2 min,P<0.05),海马CA1区锥体神经细胞死亡显著减少(P<0.05),海马神经细胞内ROS的水平也显著降低(P<0.05)。结论丙酮酸盐可能通过降低海马组织内ROS的产生,减少海马神经细胞死亡,从而提高AD模型大鼠的学习记忆水平。Objective To study the effects of pyruvate on learning, memory and neuronal cell death of the hippo- campus in a rat model of Alzheimer＇ s disease （AD）. Methods A total of 36 rats were randomly divided into control group, AD model group, and pyruvate group. General condition was observed after 6-week dipsaeus treatment. Morris water maze was used to determine learning and memory in animals.Hematoxylin and eosin （H-E） staining was used to quantify neuronal death in the hippocampus. The reactive oxygen species （ROS） was assessed by using 2＇, 7＇ -dichlorodi- hydrofluoreseein diaeetate （H2DCF-DA）. Results Compared with AD model group, pyruvate group showed improved gen- eral condition, shortened escape latency （41.37±6.24, 31.67±7.26, 24.26±7.16, 20.45±5.72, 19.36±5.89, respectively P 〈 0.05）,increased activity time on the former platform quadrant （50.36±7.46 s, P 〈 0.05）,increased number of crossing the former platform （3.98±1.23 time/2 min, P 〈 0.05）,reduced ROS levels and decreased neuronal cell death in CA1 of the hippocampus decreased （respectively, P〈0.05）. Condusions Pyruvate can improve the learning and memory and de- crease the neuronal cell death through preventing the ROS production in AD rats.

关 键 词：丙酮酸盐 阿尔茨海默病 学习记忆 

分 类 号：R749.16[医药卫生—神经病学与精神病学]