三颗针有效成分盐酸小檗碱精制工艺的优化  

Optimization of Refining Process of Berberine Hydrochloride from Berberis Pruinosa Fr.

作　　者：朱兰寸[1] 李俊平[1] 聂立勇 王林玲[1] 何雪君[1] 廖伊茜 马廷升[1] 

机构地区：[1]怀化医学高等专科学校药学系,湖南怀化418000

出　　处：《中国药业》2014年第7期38-39,共2页China Pharmaceuticals

基　　金：湖南省教育厅实验教学改革基金资助项目;项目编号:[2010]243(515);湖南省大学生研究性学习和创新性项目[2013]191(565)

摘　　要：目的研究三颗针有效成分盐酸小檗碱的最佳精制工艺。方法采用厶（3。）正交试验设计，以提取率为考察指标筛选精制工艺。结果优选得到最佳精制工艺条件为50倍样品纯化水用量、回流时间30min、溶液pH为1～2、乙醇浓度70％。三颗针有效成分盐酸小檗碱的精制提取率平均为值75．7％，RSD为2．65％（n=5）。结论由，L9（3^4）试验设计筛选得到三颗针有效成分盐酸小檗碱的精制工艺条件可靠，为该原料药的生产提供参考。Objective To study the best refining process of berberine hydrochloride from berberis pruinosa Fr. Methods With the ex- traction yield as the investigation index,the L9（34） orthogonal test was adopted to screen the refining process of berberine hydrochloride from berberis pruinosa Fr. Results The optimized best refined process conditions were as follows：purified water dosage for 50 times of samples,the reflux time for 30 min,solution pH was 1 -2,and ethanol concentration of 70%. The average extraction yield of the effec- tive ingredient berberine hydrochloride from berberis pruinosa Fr. was 75.7%, RSD 2.65% （n =5）. Conclusion The refining process of berberine hydrochloride from berberis pruinosa Fr. screened by L9（3^4） test design is reliable and provides reference for the production of this raw material drug.

关 键 词：三颗针 盐酸小檗碱 精制工艺 

分 类 号：TQ461[化学工程—制药化工] R282.71[医药卫生—中药学]