天然水和土壤中ng/L水平亚硒酸态、硒酸态和腐植酸结合态硒的分离与测定  

Determination of Selenite,Selenate and Humic Substance-bound Selenium in Natural Waters and Soil Extracts at Ng / L Levels

作　　者：李晋[1] 王大成[1] 刘丹[1] 刘燕[1] 

机构地区：[1]西华师范大学化学化工学院,化学合成与污染控制四川省重点实验室,四川南充637009

出　　处：《西华师范大学学报（自然科学版）》2014年第1期36-40,共5页Journal of China West Normal University(Natural Sciences)

基　　金：国家自然科学基金项目(No.41172326)

摘　　要：天然水和土壤提取液中的亚硒酸态硒和硒酸态硒用Dowex 1X8树脂填充色谱柱分离,腐植酸结合态硒用Supelite XAD-8树脂色谱柱分离,经酸消解后以2,3-二氨基萘荧光分光光度法测定.水样中总硒、亚硒酸态硒、硒酸态硒和腐植酸结合态硒的检出限分别为7.36,9.12,3.60和6.69 ng/L,分别用每100mL加入50 ng总硒、亚硒酸态硒和硒酸态硒的超纯水测定其回收率为95.1%-104.1%(n=5).测定河水总硒、亚硒酸态硒、硒酸态硒和腐植酸结合态硒的变异系数分别为2.11%,4.76%,4.20%和6.93%(n=5),各形态的河水样品加标回收率为95.1%-102.6%.A method for accurately analyzing total selenium （Se）,selenite （SeO32-）,selenate （SeO42-） and humic substance-bound Se in natural waters and soil extracts at ppt levels using fluorometry was developed.The separation of SeO32-and SeO42-was achieved through Dowex 1X8 resin column while the separation of humic substancebound Se was done by SupeliteTM XAD-8 column.The separated species and the total Se were acid digested and measured by 2,3-diaminonapthalane fluorometry.The detection limit（3,n =10） for total Se,SeO32-,SeO42-,and humic substance-bound Se were 7.36,9.12,3.60,and 6.69ng/L,respectively.The accuracy of the method was tested by using 50ng of total Se,SeO32-,and SeO42-standard added 100mL solutions,and the results showed a recovery of 95.1-104.1％ （n =5）.The coefficient of variation of 5 replicate river water for the four analytes were 2.11,4.76,4.20 and 6.93％,respectively.For river water sample,10ng of total Se,SeO32-,SeO42-was spiked and analyzed,and the recovery was 95.1-102.6％ （n =5）.

关 键 词：水 土壤 亚硒酸 硒酸 腐植酸 形态 

分 类 号：X132[环境科学与工程—环境科学]