鹿衔草挥发油诱导人软骨肉瘤细胞SW1353凋亡的机制  

Study on mechanism of pyrolae herb essential oil inducing apoptosis in human chondrosarcoma SW1353 cells

作　　者：蔡亮亮[1] 余方荣[1] 陈加守[1] 李会婷[1] 叶蕻芝[2] 林燕萍[2] 刘献祥[2,3] 

机构地区：[1]福建中医药大学药学院,福州350122 [2]福建中医药大学中西医结合研究院,福州350122 [3]福建省中西医结合老年性疾病重点实验室,福州350122

出　　处：《中华中医药杂志》2014年第4期1085-1088,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：陈可冀中西医结合发展基金(No.CKJ2011003)~~

摘　　要：目的:探讨鹿衔草挥发油诱导人软骨肉瘤细胞SW1353凋亡的作用机制。方法:MTT法检测鹿衔草挥发油对人软骨肉瘤细胞SW1353增殖的抑制作用;流式细胞仪检测细胞凋亡;RT-PCR检测Bcl-2,Bax基因的表达;Western Blot检测Bcl-2蛋白的表达。结果:鹿衔草挥发油在50μg/mL-150μg/mL浓度范围内能明显抑制SW1353细胞的增殖,并呈剂量和时间依赖性,IC50为106μg/mL;流式细胞仪检测的结果显示鹿衔草挥发油能诱导SW1353细胞凋亡;RT-PCR结果表明Bcl-2基因表达降低,Bax基因表达增强;Western Blot结果表明Bcl-2蛋白表达降低。结论:鹿衔草挥发油通过诱导细胞凋亡抑制SW1353细胞增殖,可能与其下调Bcl-2表达,上调Bax表达有关。Objective： To investigate the effect of pyrolae herb essential oil （PHEO） on the human chondrosarcoma cells SW1353 and study the underlying mechanism. Methods： The viability of SW1353 cells was determined by MTT assay. Flow cytometry with annexin-V/propidium was performed to determine apoptosis. The mRNA expression of Bcl-2 and Bax was measured by reverse transcription polymerase chain reaction （RT-PCR）. The protein expression of Bcl-2 was detected using Western Blot. Results： After treatment with 50l.tg/mL-1501ag/mL of PHEO, PHEO reduced viability and induced apoptosis of SW1353 cells in a dose- and time- dependent manner, the 50% inhibition concentration （ICs0） was 1061ag/mL. The result showed that PHEO treatment reduced Bcl-2 mRNA expression and enhanced Bax mRNA expression in SW1353 cells, and the protein expression of Bcl-2 was reduced versus control group. Conclusion： PHEO inhibites the growth of SW1353 cells by inducing cancer cell apoptosis, which may be associated with up-regulation of Bax expression and down-regulation of Bcl-2 expression.

关 键 词：鹿衔草 挥发油 SW1353细胞 细胞凋亡 BCL-2 BAX 

分 类 号：R738.3[医药卫生—肿瘤]