胃癌新鲜离体组织的傅里叶变换红外光谱研究  

Detection of freshly removed gastric cancer tissues by Fourier Transform Infrared Spectroscopy

作　　者：刘铭[1] 白月奎[1] 余力伟[1] 刘凯东[1] 付静[2] 冷慧[2] 张元福[3] 祝青[3] 李文斌 

机构地区：[1]北京市海淀医院普外科,北京100080 [2]北京市海淀医院病理科,北京100080 [3]北京大学化学与分子工程学院,北京100871 [4]山西省运城市中心医院肿瘤科,运城044000

出　　处：《国际外科学杂志》2014年第3期184-187,共4页International Journal of Surgery

基　　金：北京市海淀区自然科学技术委员会资助项目（No.K20110100X）

摘　　要：目的 通过对胃癌新鲜离体组织进行傅里叶变换红外光谱测定,探讨其区分胃癌组织和正常胃组织的可行性及机制.方法 联合应用衰减全反射探头及中红外光纤、傅里叶变换红外光谱仪,检测胃癌手术中切除的新鲜离体胃癌组织50例.比较胃癌组织和正常胃组织红外光谱各谱带的相对峰位、相对峰强.结果 与蛋白、脂质、糖类和核酸相关的谱带在胃癌组织和正常胃组织之间差异明显具有统计学意义（P＜0.05）.结论 傅里叶红外光谱技术可区别新鲜离体状态下胃癌组织和正常胃组织,联合应用衰减全反射探头及中红外光纤、傅里叶变换红外光谱仪有望成为胃癌诊断及术中导航的新方法.Objective To identify the FTIR spectroscopic characterization of breast cancer and explore the possibility of application of FTIR in differentiation of malignant and benign gastric lesions.Methods FTIR spectra of surgically removed fresh gastic tissues were measured by spectrometer equipped with mid-infrared fiber optics and an ATR probe.Peaks in the spectra were measured and relative intensity ratios were calculated and analyzed if there are significant differences between the spectra of malignant and benign gastic lesions.Results There were significant differences between the spectra of malignant gastic cancers and benign gastic tissues in protein structures 、lipid structures、carbohydrate and nucleic acid.Conclusions FTIR spectroscopy could be a useful tool in clinical diagnosis of gastic cancer and new methods of intraoperative navigation.

关 键 词：傅里叶变换红外光谱 胃肿瘤 腺癌 新鲜离体组织 

分 类 号：R735.2[医药卫生—肿瘤]