甘氨酸对重症急性胰腺炎大鼠肺损伤保护机制的研究  

Effect of glycocine on acute lung injury of the severe acute pancreatitis in rats

作　　者：陈伟峰[1] 周丹[1] 熊丽红[1] 林明琴[2] 

机构地区：[1]深圳市第二人民医院急诊ICU,广东深圳518028 [2]海南医学院药学院药理学教研室,海南海口571101

出　　处：《海南医学院学报》2014年第5期599-601,604,共4页Journal of Hainan Medical University

基　　金：深圳市科技计划资助项目(201003032)~~

摘　　要：目的:探讨甘氨酸对重症急性胰腺炎大鼠肺损伤的保护作用机制。方法:将SD大鼠72只随机分成假手术组(A组)、重症急性胰腺炎组(B组)、甘氨酸组(C组)。重症急性胰腺炎模型通过胰胆管逆行注射5%牛磺胆酸钠诱导,甘氨酸组(C组)于造模前3、6h分别从尾静脉内注入甘氨酸(3mmol/L,0.1mL/100g)预处理,各组在造模完成后分别于6、12、24h处死动物取其肺组织,采用酶联免疫吸附法(ELISA)测定肺组织高迁移率族蛋白B-1(HMGB-1)含量,采用RT-PCR法测定肺组织髓样细胞触发受体-1(TREM-1)mRNA水平。结果:与A组比较,B组肺组织HMGB-1水平、TREM-1mRNA的表达明显增高,差异均有统计学意义P<0.05);与B组相比,C组HMGB-1水平、TREM-1mRNA的表达呈下降趋势,差异均有统计学意义(P<0.05)。结论:甘氨酸可以抑制重症急性胰腺炎肺组织中TREM-1mRNA及HMGB-1的表达,从而对重症急性胰腺炎肺损伤起保护作用。Objective： To investigate the effect of glycocine on acute lung injury induced by the severe acute pancreatitis in rats. Methods： The rats were randomly divided into three groups： sham operation rats （group A）, severe acute pancreatitis group （group B） and SAP rats given the glycocine（group C）. The SAP model was established by injecting 5% sodium taurocholate into biliopancreatic ducts with needle. The glycocine group was injected with glycocine through vena caudalis at 3 h, 6 h before the SAP model induced. Rats in each group were sacrificed at 6 h, 12 h, 24 h after sham operation or SAP. The concentrations of HMGB-1 were measured by ELISA, TREM-1 mRNA expressions were measured by RTPCR. Results： Compared with group A, the levels of HMGB-1 and the expressions of TREM-1 mRNA in group B were significantly elevated （P〈0.05）. In group C, these items were lower than that of group B （P〈0.05）. Conclusions： Glyine can decrease the expression of TREM-1 mRNA and HMGB1, then can be used for treatment of severe acute pancreatitis.

关 键 词：甘氨酸 髓样细胞触发受体-1(TREM-1) 高迁移率族蛋白B-1(HMGB-1) 重症急性胰腺炎 肺组织 

分 类 号：R-332[医药卫生]