肝豆灵对铜负荷肝纤维化大鼠肝星状细胞凋亡的影响  

Effect of Gandouling on Apoptosis of Hepatic Stellate Cells in Copper-Overloaded Rats with Hepatic Fibrosis

作　　者：张娟[1] 谢道俊[1] 韩辉[1] 沈斌[2] 鲍远程[1] 童建兵[1] 周磊[1] 杨波[1] 丁伟军[1] 

机构地区：[1]安徽中医药大学第一附属医院神经内科,安徽合肥230031 [2]湖北中医药大学,湖北武汉430065

出　　处：《安徽中医药大学学报》2014年第2期63-66,共4页Journal of Anhui University of Chinese Medicine

基　　金：国家自然科学基金项目(81202692);安徽高校省级自然科学研究项目(KJ2012Z223)

摘　　要：目的观察肝豆灵对铜负荷肝纤维化大鼠肝星状细胞(hepatic stellate cells,HSC)凋亡的影响,探讨肝豆灵防治Wilson病肝纤维化的作用机制。方法将75只大鼠分成空白组、模型组、肝豆灵组、青霉胺组及肝豆灵+青霉胺组,采用喂食硫酸铜饮食复制铜负荷大鼠模型,采用TUNEL法检测HSC的凋亡。结果模型组可见大量HSC增生;与模型组比较,各治疗组HSC数量显著降低(P<0.01),HSC凋亡指数显著升高(P<0.01);肝豆灵+青霉胺组AI显著高于肝豆灵组和青霉胺组(P<0.01)。讨论肝豆灵可促进铜负荷大鼠HSC的凋亡,其与青霉胺联合用药的作用更显著。Objective To observe the effect of Gandouling on the apoptosis of hepatic stellate cells （HSCs） in copper-overloaded rats with hepatic fibrosis and to investigate the action mechanism of Gandouling in the prevention and treatment of hepatic fibrosis in Wilson＇s disease. Methods Seventy-five rats were divided into blank group, model group, Gandouling group, penicillamine group, and Gandouling plus penieillamine group. All rats except those in blank group were given copper sulfate-containing feed to induce a copper-overloaded rat model. The apoptosis of HSCs was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling. Results The model group had extensive proliferation of HSCs. Compared with the model group, all treatment groups had significantly reduced numbers of HSCs （P〈0.01） and significantly increased apoptotic indices （AIs） （P〈0.01） ; the Gandouling plus penicillamine group had a significantly higher AI than the Gandouling group and penicillamine group （P〈0.01）. Conclusion Gandouling can promote the apoptosis of HSCs in copper-overloaded rats, and it has better efficacy when used in combination with penicillamine.

关 键 词：肝豆状核变性 肝纤维化 铜负荷 肝星状细胞 肝豆灵 青霉胺 

分 类 号：R575.24[医药卫生—消化系统]