三羟异黄酮对HER2/neu高表达人乳腺癌细胞辐射敏感性的影响  

Effect of Genistein on Radiosensitivity of Breast Cancer Cells with High Expression of HER2/neu

作　　者：李明[1] 周永[2] 谭宗才 徐敏[1] 郑钧丰[1] 常蕾[1] 张林[1] 屈琳[1] 糜漫天[2] 

机构地区：[1]成都军区疾病预防控制中心,成都610021 [2]第三军医大学军事预防医学院营养与食品卫生学教研室 [3]广州军区综合训练基地二大队

出　　处：《西南国防医药》2014年第4期358-361,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：重庆市自然科学基金(CSTC 2006 BB 5103)

摘　　要：目的探讨三羟异黄酮对人乳腺癌细胞株MDA-MB-453(ER-,HER2-high)、MCF-7/HER2(ER+,HER2-high)和MCF-7(ER+,HER2-low)辐射敏感性的影响,并观察三羟异黄酮对HER2/neu高表达人乳腺癌细胞辐射敏感性的作用。方法通过基因转染技术建立HER2/neu高表达的人乳腺癌MCF-7细胞,免疫荧光细胞化学法鉴定;经100 mol/L三羟异黄酮联合或单独10 Gyγ射线处理MDA-MB-453、MCF-7/HER2和MCF-7三种乳腺癌细胞24 h后,采用流式细胞仪检测三羟异黄酮对乳腺癌细胞辐射敏感性的影响。结果与对照组相比,MDA-MB-453、MCF-7/HER2细胞的射线组凋亡率无显著差异(P>0.05),三羟异黄酮联合射线组的凋亡率,与射线组和对照组相比,分别增加25.51%(P<0.05)、56.16%(P<0.05);MCF-7细胞射线组凋亡率比对照组增加4.62%(P<0.05),而三羟异黄酮联合射线组与射线组比较没有显著差异(P>0.05)。结论 HER2/neu高表达的乳腺癌细胞对射线诱导的凋亡有抵抗作用,但三羟异黄酮可增强γ射线诱导HER2/neu高表达乳腺癌细胞MDA-MB-453细胞、MCF-7/HER2细胞的凋亡作用。Objective To investigate the effects of genistein on the radiosensitivity of human breast cancer cell strains with expression of MDA-MB-453 （ ER-, HE R2-high）, MCF-7 （ ER ＋, HER2-high）, and MCF-7/HER2 （ ER ＋, HER2-1ow）, and to observe the effects of genistein on the radiosensitivity of HER2/neu highly expressed human breast cancer cells. Methods Highly expressed HER2/neu human breast cancer cell MCF-7 was constructed through cell transfection technology and was identified by immunofluorescence cytochemical technology. After the three kinds of breast cancer cells i. e. MDA-MB-453, MCF-7/HER2, and MCF- 7 were treated by 100 μmol/L genistein combined with or simply use of 10 Gy γ ray,flow cytometry was used to analyze the effects of genistein on the radiosensitivity of the breast cancer cells. Results There was no significant difference between the apoptotic rates of MCF-7/HER2 and MDA-MB-453 cells treated by γ ray compared with those of the control group （ P 〉 0.05 ）. The apoptotic rates of those cells treated by genistein combined with γ ray increased by 25.51% （ P 〈 0.05 ） and 56.16% （ P 〈 0.05 ）, respectively, compared with those of the cells treated only by γ ray and the control group. The apoptotic rate of MCF-7 cells treated by γ, ray increased by 4.62% （P 〈0.05）compared with that of the control group（P 〈 0. 05）,but there was no significant difference between the apoptotic rates of the cells in the γ ray and the control group （ P 〉 0.05 ）. Conclusions HER2/neu highly expressed breast cancer cells have resistant effect on the apoptosis induced by γ ray,but genistein can increase the apoptotic effect of HER2/neu highly expressed human breast cancer cells induced by γ ray.

关 键 词：三羟异黄酮 人乳腺癌细胞 辐射敏感性 MCF-7 HER2 MDA-MB-453 

分 类 号：R737.9[医药卫生—肿瘤]