酒精性肝纤维化大鼠肝组织中TGF-β1/Smads信号分子表达的变化  

The changing pattern of TGF- β1 / Samds pathway in alcoholic liver fibrosis of rats

作　　者：张天奇 何伟莉[2] 

机构地区：[1]庆元县人民医院内科,浙江庆元323800 [2]丽水市中心医院消化科,浙江丽水323000

出　　处：《健康研究》2014年第2期136-138,共3页Health Research

摘　　要：目的观察TGF-β1/Smads信号分子在酒精性肝纤维化大鼠肝组织中的变化,探讨酒精性肝纤维化的发病机制。方法 50只SD大鼠,随机分成模型组和对照组各25只。模型组用56度白酒灌胃(15ml.kg-1.d-1)致肝纤维化,对照组以等量生理盐水代替灌胃。6个月后全部处死取肝待检。采用HE染色观察肝纤维化程度,免疫组化法观察肝组织中TGF-β1蛋白的表达水平,RT-PCR法观察肝组织中TGF-β1和Smads mRNA的表达水平。结果 6个月酒精灌胃能成功制作大鼠酒精性肝纤维化模型。和对照组相比较,模型组肝组织中TGF-β1蛋白表达水平显著升高(P<0.01),TGF-β1,smad3,samd4 mRNA表达水平显著升高(均P<0.01),而smad7 mRNA水平显著降低(P<0.05)。结论 TGF-β1/Smads信号通路参与酒精性肝纤维化的形成过程,其中Smad7起着对肝纤维化负调控的作用。Objective To recognize the changing patterns of TGF-β1/Samds pathway in alcoholic liver fibrosis of rats so as to understand the partial pathogenesis of alcoholic liver disease. Methods 50 healthy SD rats were selected and divided into an experimental group and a control group randomly. 25 rats of the experimental group were given a dosage of 15ml· kg-1 d-lof 56°alcohol for six months, while the 25 rats of the control group were given normal saline instead but of the same amount. All rats were sacrificed after six months and their liver specimens were taken out for examination. We applied HE method to detect the degree of rats＇ alcoholic liver fibrosis, imumno-histo-chemistry examination to detect the protein expression and distribution of TGF-fll , and RT-PCR method to detect the mRNA expression of TGF-β1 and Smads. Findings Alcoholic liver fibrosis rat model was successfully made by 56° alcohol-garage after six months. There was significant differences in liver fibrosis between the control group and the experimental group（ P 〈 0.01 ）. Compared with thecontrol group, the TGF-β1 protein of the experimental group significantly increased （P 〈 O. 01 ）, and was mainly distributed in portal area, peri-sinusoidal space and fibrous septa. The mRNA expression of TGF-β1, smad3 and samd4 were higher in rats of the experimental group compared with those of the control group （ （ P 〈 O. O1 ,P 〈 0.01 ,P 〈 0.01 ）. However, the smad7 mRNA decreased significantly in model rats （P 〈 O. 05）. Conclusion There appeared significant changes of the expression of TGF-β1/Smads in rats of the experimental group compared with those of the control group, wherein, TGF-β1, smad3 and samd4 were up-regulated while smad7 was down-regulated. The abnormal change of TGF-β1/Smads pathway might play an important role in the development of Alcoholic Liver Fibrosis Disease.

关 键 词：酒精性肝纤维化 转化生长因子Β1 SMADS 

分 类 号：R57[医药卫生—消化系统]