鹅细胞色素P450 2C45(CYP2C45)基因多态性及其与产肝性能的关联分析  

Association Analysis of Polymorphism in the CYP2C45 Gene with Fatty Liver Production in Geese

作　　者：张蕊[1] 张宜辉[1] 孙晓先[1] 龚道清[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国畜牧兽医》2014年第4期180-184,共5页China Animal Husbandry & Veterinary Medicine

基　　金：高等学校博士学科点专项科研基金(博导类)(20133250110007);国家自然科学基金(31372298);江苏省科技支撑项目(BE2011328);江苏省农业科技自主创新资金(CX(12)2033)

摘　　要：为了研究细胞色素P450 2C45(CYP2C45)基因上的遗传变异对朗德鹅产肝性能的影响,试验利用PCR结合测序技术检测113只朗德鹅CYP2C45基因外显子上的SNPs,并与产肝性能进行关联分析。结果表明,在CYP2C45的外显子上共发现4个SNPs,分别为位于外显子5上111bp处的C>T突变和147bp处的C>T突变,以及外显子7上71bp处的A>G突变和135bp处的G>A突变。连锁不平衡分析发现,这4个突变位点处于完全连锁状态,将这4个SNPs合并作为一种基因型,可分为AA基因型(CCAG/CCAG)和AB基因型(CCAG/TTGA),但并未发现BB基因型(TTGA/TTGA),AA和AB基因型频率分别为0.9027和0.0973,A和B的基因频率分别为0.9513和0.0487。对不同基因型朗德鹅的屠体重、肝重和肝体比进行方差分析,发现AA基因型的屠体重显著低于AB基因型(P<0.05),AA基因型的肝重和肝体比虽然高于AB基因型,但均未达到显著水平(P>0.05)。因此,在产肝量相当的情况下,体重越小,对饲料的消耗就越小,可以在一定程度上节约成本,有助于生产效益的提高。In order to study the influence of genetic variation in CYP2C45 gene on fatty liver production performance, PCR and sequencing methods were used to identify polymorphism of CYP2C45 gene exons in 113 Landes goose, and the association of polymorphism with production performance of goose fatty liver were investigated. The results indicated that there were 4 SNPs in exon 5 at 111 bp of C〉T transition and at 147 bp of C〉T transition, and in exon 7 at 71 bp of A〉G transition and at 135 bp of G〉A transition, respectively. These SNPs were in the stage of complete linkage by linkage disequilibrium analysis. Combining 4 SNPs as one genotype, there were AA （CCAG/CCAG） and AB （CCAG/TTGA） genotypes, but BB genotype （TTGA/TTGA） was not found, the genotype frequencies were 0. 9027 and 0. 0973, respectively, and the gene frequencies of alleles A and B were 0. 9513 and 0. 0487, respectively. Analysis of variance showed that the body weight of AB genotype was higher significantly than AA genotype （P〈0.05）, although the liver weight and ratio of liver weight versus body weight of AA genotype were higher than AB genotype, there were no significant difference （P〉0. 05）. It suggested that, in the case of the same yield of liver production, less body weight less feeding, AA genotype maybe contribute to the improvement of production efficiency.

关 键 词：朗德鹅 CYP2C45基因 SNP 肥肝 

分 类 号：S813.3[农业科学—畜牧学]