禽源致病性沙门氏菌耐药表型与耐药基因的分析  

Analysis of Resistant Phenotypes and Resistant Genes of Pathogenic Salmonella from Avian

作　　者：戴建华[1] 吴植[1] 袁维峰[2] 

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国畜牧兽医》2014年第4期245-248,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家高技术研究发展计划(863计划)(2012AA101302);公益性行业(农业)科研专项(200903027;201303042);中央级公益性科研院所基本科研业务费专项资金项目(2011js-3);农业部动物疫情监测与防治项目

摘　　要：为了解江苏苏中地区禽源沙门氏菌耐药现象及耐药基因的存在情况，采用K—B法对22株沙门氏菌分离株进行12种抗菌药物敏感性试验，并通过PCR方法检测了12种耐药基因的存在情况。结果显示，所有菌株对甲氧胺嘧啶、阿米卡星、庆大霉素、萘啶酮酸、诺氟沙星的耐药率均在72．73％～100％之间；存在着5种耐药表型，每个耐药表型均在4重或4重以上，耐药最多菌株可耐受10种药物；扩增出9种耐药基因，与GenBank中的参考序列同源性均在98％以上；耐药表型与耐药基因的存在基本一致，但无绝对相关性。本研究结果表明苏中地区沙门氏菌耐药现象非常严重，且耐药基因广泛存在。To investigate the presence of antibiotic resistance and resistant genes about avian Salmonella in Suzhong area, 22 isolates were evaluated for antimicrobial sensitivity by K-B disc diffusion method against 12 antimicrobial drug, and then 12 resistant genes of the isolates were detected by PCR. The resistant rates of all isolates were found from 72.73% to 100% to amikacin, norfloxacin and so on; 5 resistant phenotypes were detected, and each isolates were resistant to more than 4 of the 12 antimicrobial drugs,even 10 drugs; 9 different resistant genes were amplified, the homologies were very high compared with the corresponding genes in GenBank （≥98%）; the resistant phenotypes and their resistance related genes were essential con- sistent, but no absolute correlation. The above results showed that the antibiotic resistance of Salmonella was very serious, and the resistant genes existed widely.

关 键 词：禽 沙门氏菌 耐药表型 耐药基因 

分 类 号：S852.61[农业科学—基础兽医学]