小儿败血症16S rRNA基因检测及DNA分析  

Detection and DNA analysis of 16S rRNA gene in children with septicemia

作　　者：刘愉[1] 李丽红[1] 孙利炜[1] 高玉堂[1] 宇亚娟[1] 贺岩[1] 邓琳菲[1] 赵艳玲[1] 王承训[1] 

机构地区：[1]吉林省长春市儿童医院,130051

出　　处：《中国妇幼保健》2014年第12期1915-1918,共4页Maternal and Child Health Care of China

基　　金：长科技合〔2008118〕

摘　　要：目的：提高小儿败血症病原学的早期诊断。方法：采用细菌16SrRNA基因通用引物法对临床诊断重症感染患儿血液标本进行聚合酶链反应（PCR扩增），并与细菌培养结果进行比较，同时分析其临床表现。结果：共检测182例感染性疾病患儿血液标本，其中33例16SrRNA基因检测阳性，阳性率18．13％；血培养阳性26例，阳性率14．29％。细菌16SrRNA基因PCR扩增方法灵敏度高、特异性好，实验周期为5～6h，明显优于细菌培养方法。结论：细菌16SrRNA基因PCR扩增方法为重症感染患儿败血症的诊断提供了一种快速的病原学诊断依据。Objective： To improve early etiological diagnosis of children with septicemia. Methods： 165 rRNA gene segment in the blood specimens of children diagnosed as severe infection were amplified by 16S rRNA gene universal primers, then the results were com- pared with results of bacterial culture, the clinical manifestations were analyzed simuhaneously. Results： The blood specimens from 182 children with infectious diseases were detected, and 33 children were found with positive 16S rRNA gone, the positive rate was 18.13% ; 26 children were found with positive 16S rRNA gene by blood culture, the positive rate was 14. 29%. The specificity and sensitivity of PCR am- plification in bacterial 16S rRNA gene were high, the experimental cycle was 5 -6 hours, which was significantly superior to bacterial cul- ture. Conclusion： Bacterial 16S rRNA gene amplification with PCR provides a quick etiological basis for diagnosing septicemia in children.

关 键 词：小儿 感染性疾病 细菌 16S RRNA 

分 类 号：R37[医药卫生—病原生物学]