人参内生细菌的分离及其体外抗肿瘤活性分析  

Analysis on isolation of endophytic bacteria from Panax ginseng Meyer and its antitumor activity in vitro

作　　者：曲红光[1,2] 靳慧娟[1,3] 马丽伟[1,2] 李晓宁[1,2] 谢向丽[1,2] 王冬音 李诗恒[1,2] 于慧美[1,2] 

机构地区：[1]吉林省长春市妇产医院生殖中心,130021 [2]吉林大学基础医学院病原生物学系 [3]华中科技大学同济医学院基础医学院病理生理学系

出　　处：《中国妇幼保健》2014年第12期1926-1928,共3页Maternal and Child Health Care of China

基　　金：国家自然科学基金项目〔No.81201268〕;中国博士后基金项目〔No.2013M540256〕;湖北省自然科学基金项目〔No.2012FFB03706〕

摘　　要：目的:分离6年生人参内生细菌,并初步分析其代谢产物粗提物体外抗肿瘤活性。方法:采用75%乙醇、次氯酸钠联合应用的方法消毒6年生人参,分离获得其中内生细菌,16SrRNA测序确定内生细菌的种属,利用MTT方法检测内生细菌发酵代谢产物抗肿瘤活性。结果:从6年生人参中分离得到内生细菌18株,其中有3株内生细菌代谢产物粗提物对人宫颈癌细胞Hela的IC50分别为8.4μg/ml、10.3μg/ml、11.6μg/ml,具有较好的抗肿瘤活性。在倒置光学显微镜下,可以观察到明显的肿瘤细胞皱缩等凋亡现象。结论:人参中含有丰富的内生菌资源,且其中含有抗肿瘤活性物质,这为寻找抗肿瘤药物提供了新的思路和研究方向。Objective： To isolate endophytic bacteria from six - year - old Panax ginseng Meyer and preliminarily analyze the anti- tumor activity of crude extract of its metabolites. Methods： The six- year- old Panax ginseng Meyer was disinfected by 75% ethanol and sodium hypochlorite, the endophytic bacteria were isolated and obtained, 16SrRNA sequencing was used to determine the species, MTT as- say was used to detect the antitumor activity of metabolites. Results ： Eighteen endophytie bacteria were isolated from six - year - old Panax ginseng Meyer. IC50 values of crude extracts of metabolites of three strains of Panax ginseng Meyer to human cervical can HeLa cells were 8.4 μg/ml, 10. 3 μg/ml and 11.6 μg,/ml, respectively, the antitumor activity was good; apoptosis of HeLa cells could be observed under inverted optical microscope, such as shrinkage of HeLa cells. Conclusion： Panax ginseng Meyer is rich in endophytic bacteria, and it con- tains antitumor active substances, which provide new ideas and research directions for finding anticancer drugs.

关 键 词：人参 内生细菌 体外 抗肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]