蛋氨酸脑啡肽对MCF-7/ADR生长抑制作用机制的初步研究  

Growth inhibition mechanism of Methionine Enkephalin on MCF-7/ADR

作　　者：田力[1] 刘霏霏[2] 刘泽源[2] 

机构地区：[1]海军总医院特需医疗部,北京100048 [2]军事医学科学院附属医院临床药理学研究室,北京100071

出　　处：《中国现代医学杂志》2014年第3期6-10,共5页China Journal of Modern Medicine

摘　　要：目的研究阿片生长因子蛋氨酸脑啡肽(Met-ENK)对MCF-7/ADR肿瘤细胞的抑制作用的效果及机制的初步研究。方法采用CCK-8法检测不同剂量的Met-ENK(10-3、10-4、10-5、10-6、10-7 mol/L)对乳腺癌细胞MCF-7/ADR作用48 h后的肿瘤抑制率;采用细胞计数法绘制Met-ENK孵育0、24、36、48及72 h后的MCF-7/ADR的生长曲线;采用流式细胞术及PI染色法记录用药孵育48 h后,MCF-7/ADR细胞周期变化情况。结果结果显示与空白对照组相比,Met-ENK对MCF-7/ADR细胞的生长有明显的抑制作用,呈时间依赖性,但不呈剂量依赖性,10-6mol/L浓度时,Met-ENK抑制肿瘤细胞生长效应最强;在Met-ENK作用后主要将细胞抑制于生长周期的G0/G1期。结论 Met-ENK对MCF-7/ADR肿瘤细胞有明显的抑制作用,且主要是通过影响细胞周期阻滞来发挥抑制肿瘤细胞生长的作用。但其机制仍需进一步研究及证实。[Objective] To study the growth inhibition effect and mechanism of the opioid growth factor Methionine enkephalin （Met-ENK） on MCF-7/ADR tumor cell. [ Methods ] CCK-8 assay with different doses of Met-ENK （10-3, 10-4, 10-5, 104, 10-7 mol/L） on breast cancer cell MCF-7/ADR after 48 hours for the rate of tumor suppressor. Cell counting to draw the growth curve after Met-ENK incubated 0, 24, 36, 48 and 72 h on the MCF-7/ ADR. Flow cytometry and PI staining was used to detect the cell cycle changes of MCF-7/ADR. [Results] Compared with control group, Met-ENK group significantly inhibited MCF-7/ADR cell growth in time-dependant manner instead of concentration-dependant manner. The number of MCF-7/ADR cells was the lowest after treatment with 104 mol/L Met-ENK compared with those of other concentration treatment. Met-ENK mainly inhibits the cells in the GdG~ phase of the growth cycle. [ Conclusions ] Met-ENK has inhibitory effect on MCF-7/ADR tumor cells growth mainly by causing G0/G1 phase arrest in MCF-7/ADR cells. The mechanism of Met-ENK inhibitory effect on tumor cells should be further studied and validated.

关 键 词：蛋氨酸脑啡肽 MCF-7 ADR细胞株 G0 G1期 抑制作用 

分 类 号：R737.9[医药卫生—肿瘤]