改良酸性丙酮法提取鸭血血红素的研究  

Preparation of Heme from Duck Blood by a New Acidified Acetone Extraction Method

作　　者：沈亮[1,2] 徐晓燕[3] 赵斌斌[1] 曾强成[2] 

机构地区：[1]生物技术与生物资源利用山东省高校重点实验室,生命科学学院,德州学院,山东德州253023 [2]山东行功能人分子重点实验室,德州学院,山东德州253023 [3]德州市人民医院,山东德州253014

出　　处：《中国药学杂志》2014年第8期664-668,共5页Chinese Pharmaceutical Journal

基　　金：山东省星火计划(2012XH13002);德州市科技局科技发展计划项目(2010A62-09)

摘　　要：目的提取樱桃谷肉鸭鸭血中的血红素。方法用微波辅助酸性丙酮法制备鸭血血红素。结果单因素实验,证明了微波的功率和萃取时间对整个萃取结果影响显著。设计了正交实验,实验结果显示,确定了实验室条件下微波辅助萃取的最佳工艺条件:功率为800 W,时间为60 min,温度为50℃。结论改良传统酸性丙酮法,加入微波辅助工艺将细胞破碎和萃取过程合二为一,简化其提取工艺。OBJECTIVE To prepare heme from cherry valley duck blood. METHODS Single factor experiments and orthogonal experiment were carried out to establish the optimal process of preparing heine from cherty valley duck blood by a new microwave-assis-ted acid acetone extraction method. RESULTS Single factor experiments proved that the microwave-assisted extraction process signit： icantly improved the extraction efficiency of heine. In addition, orthogonal experiment indicated the power of microwave and microwave extraction time had significant effects on the extraction results, and the optimum conditions of microwave-assisted extraction in laborato-ry were as follows ： the power was 800 W, extraction time was 60 rain, and the temperature was 50 ℃. CONCLUSION The new mi-crowave-assisted method, combining cell disruption and heine extraction process, can simplify the traditional acid acetone extraction process, and it is proved to be feasible.

关 键 词：酸性丙酮法 微波萃取 鸭血 血红素 

分 类 号：R944[医药卫生—药剂学]