基于均匀设计法优化土沉香的组培再生体系研究  

Study on Uniform Design Methods for Optimization of Plant Regeneration of Aquilariasinensis(Lour.)Spreng.in vitro

作　　者：贾贤[1,2] 贾瑞[1,2] 王颖[2] 吴坤鑫[2] 陈雄庭[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《北方园艺》2014年第8期100-104,共5页Northern Horticulture

基　　金：海南省重大科技攻关资助项目(ZDZX2013023-1)

摘　　要：以带有腋芽的幼嫩土沉香茎段为外植体,运用DPS数据处理系统,通过均匀设计法筛选诱导土沉香茎段从出芽到生根整个再生体系的适宜培养基。结果表明:适宜土沉香茎段腋芽萌发的培养基为7/10MS+6-BA 0.35mg/L+NAA 0.01mg/L,诱导率为93.33%;适宜土沉香出芽增殖的培养基为7/10MS+KT 1.27mg/L+NAA 0.2mg/L,增殖倍数为3.53;适宜土沉香茎段生根的培养基为7/10MS+6-BA 0.02mg/L+IBA 1.23mg/L+CM 25mL/L,诱导率为33.33%。该试验优化了土沉香茎段再生体系的培养条件,为其快速繁殖的推广及遗传转化体系的建立提供了基础支持。Taking the stems of Aquilaria sinensis （bur.） Spreng. as explants,using DPS, the whole system for shoot regeneration were established through uniform design experiments. The results showed that the medium of 7/10 MS＋ 6-BA 0. 35 mg/L＋NAA 0. 01 mg/L was suitable for buds induction,and the rate of starting was 93. 330//00 ;the medium of 7/10 MS＋KT 1.27 mg/L＋NAA 0. 2 mg/L was suitable for bud multiplying,and the multiplying coefficient was 3. 53; the rooting medium which was 7/10 MS＋6-BA 0. 02 mg/L＋ IBA 1.23 mg/L＋CM 25 mL/L was suitable with the rate of induction of 33. 33%. The medium supplement for regeneration system of Aquilaria sinensis （I.our.） Spreng. was optimized,which provides a basis support for its propagation and construction of genetic transformation system.

关 键 词：土沉香 组织培养 均匀设计 优化 

分 类 号：S772.37[农业科学—森林工程]