七彩红竹查尔酮异构酶基因IhCHI1的克隆与表达分析  

Cloning and Expression Analysis of IhCHI1 from Indosasa hispida cv. Rainbow'

作　　者：王晨晨[1] 毕玮[2,3,4] 王娟[2,3,4] 周旭[1] 杨宇明[4] 王毅[2,3,4] 

机构地区：[1]西南林业大学,云南昆明650224 [2]国家林业局重点开放性实验室云南珍稀濒特森林植物保护和繁育实验室 [3]云南省森林植物培育与开发利用重点实验室,云南昆明650201 [4]云南省林业科学院,云南昆明650201

出　　处：《西部林业科学》2014年第2期85-90,共6页Journal of West China Forestry Science

基　　金：云南省应用基础研究重点项目(2013FA054);云南省自然科学基金面上项目资助研究(2009CD073);云南省中青年学术技术带头人后备人才培养项目(2010CI016)

摘　　要：查尔酮异构酶(CHI)是花青素生物合成过程中重要的结构基因。本项实验根据转录组数据设计的特异引物,利用RT-PCR方法成功从七彩红竹红色幼茎中克隆到七彩红竹的查尔酮异构酶基因IhCHI1(GenBank登录号为KJ477333)。序列分析结果表明,IhCHI1包含完整的cDNA开放阅读框(ORF),由690 bp组成,编码229个氨基酸。Blast比对结果显示,该蛋白属于CHI家族蛋白;系统进化分析结果显示,七彩红竹与禾本科植物水稻、玉米等亲缘关系较近;利用RT-PCR方法对IhCHI1基因在不同组织中表达情况进行分析,结果表明,IhCHI1在七彩红竹的红色幼秆中表达量最高。Chalcone isomerase（ CHl）is one of the important structure genes in Anthocyanins biosynthesis. IhCHI1 was successfully obtained from lndosasa hispida cv. ＇ Rainbow＇ by RT-PCR with special primers designed based on the transeriptome data, and this sequence accession number is KJ477333 in GenBank. Sequence analysis showed that IhCHI1 had complete eDNA open reading frame （ORF） , which consisted of 690 bp encoding 229 amino acids. Blast analysis showed that IhCHI1 belonged to the CHI family. Phylogenetic analysis showed that Indosasa hispida ev. ＇ Rainbow＇ had close relative rationship with Oryza sativa and Zea mays. IhCHI1 expression analysis in different organs of Indosasa hispida cv. ＇ Rainbow＇ by RT-PCR showed that IhCHI1 strongly expressed in young red eulm.

关 键 词：七彩红竹 基因克隆 查尔酮异构酶 表达分析 

分 类 号：S795.9[农业科学—林木遗传育种]