辣根过氧化氢酶电极的研究  

Determination of Hydroquinone with HRP Modified Electrode

作　　者：高孟姣[1] 应太林[1] 漆德瑶[1] 

机构地区：[1]上海大学环境科学与工程学院,上海200072

出　　处：《分析试验室》2001年第1期55-57,共3页Chinese Journal of Analysis Laboratory

摘　　要：以辣根过氧化氢酶修饰电极采用循环伏安法对环境污染物对对苯二酚进行了测定 ,对测定条件进行了探索 ,该电极在 p H7,H2 O2 浓度为 1 0μmol· L-1时对苯二酚具有良好的响应 ,其线性范围为 2 .0× 1 0 -5～ 2 .8× 1 0 -3mol·L-1,检测下限为 1 .0× 1 0 -6mol· L-1。电极的峰电流与扫描速度的平方根成正比 ,电极上的传质过程受扩散控制 ,对酶催化反应的动力学机制进行了探讨。Horseradish peroxidase (HRP) modified electrode was successfully used to determine hydroquinone by cyclic voltammetry. The optimum determination condition was investigated with pH7 and 10 μmol·L -1 H 2O 2. The linear relationship is held from 2.0×10 5～2 8×10 -3 mol·L -1 of hydroquinone with detection limit of 1.0×10 -6 mol·L -1 . The electrode has high response speed, good reproducibility and stability. The peak current is proportional to square root of scan rate, which indicated that the procedure is controlled by diffusion. The dynamic mechanism of the enzyme catalyzed reaction was discussed.

关 键 词：辣根过氧化氢酶 对苯二酚 循环伏安法 修饰电极 污染物分析 

分 类 号：X132[环境科学与工程—环境科学]