大蒜素对大鼠心室肌间隙连接蛋白43表达水平的影响  

Effects of allicin on the expression of connexin43 isoforms in hyperlipemic rat's cardiac muscle

作　　者：刘富[1] 

机构地区：[1]哈尔滨医科大学大庆校区,黑龙江大庆163319

出　　处：《华西药学杂志》2014年第2期146-148,共3页West China Journal of Pharmaceutical Sciences

基　　金：黑龙江省卫生厅科学技术研究项目(编号:2011-239)

摘　　要：目的通过检测大蒜素治疗前后高脂血症大鼠心肌间隙连接蛋白43的表达,阐述大蒜素降血脂作用的分子机制。方法将50只Wistar大鼠均分为正常组、模型组、大蒜素高、中、低(100、50、25 mg·kg-1·d-1)剂量组,建立大鼠高脂血症模型。从尾静脉取血,测血清中的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、游离脂肪酸(NEFA)等血脂指标;利用RT-PCR技术、免疫组化方法和免疫印迹技术测定间隙连接蛋白43在正常、模型、给药3种情况下的差异。结果高脂血症大鼠血清中的TC、TG、LDL、NEFA含量升高(P<0.05),HDL含量降低(P<0.05),经大蒜素治疗后TC、TG、LDL、NEFA含量降低(P<0.05),HDL含量升高(P<0.05);高脂组与正常组比较,间隙连接蛋白43mRNA和蛋白水平显著减弱(P<0.05),给药组与高脂组比较间隙连接蛋白43mRNA和蛋白水平显著增强(P<0.05);免疫组化结果表明:高脂组与正常组比较,间隙连接蛋白43表达显著减少(P<0.05),给药组与高脂组比较其表达显著增加(P<0.05)。结论大蒜素具有降血脂作用,其降血脂的分子机制与间隙连接蛋白43的表达改变有一定联系。OBJECTIVE To find the molecular mechanism of decreasing blood fat effect of allicin on hyperlidemic rat, we studied the expression of connexin43 in the myoeardium before and after using the alliein. METHODS 50 Wistar rats were randomly divided into 5 groups ： control group, model group, allicin group of high dose, middle dose and low dose （ 100,50,25 rag. kg-1 . d-1 ）. Each group had 10 rats. Hyperlidemic rat model was established firstly, the blood which was obtained via vena caudalis and the indexes of TC,TG,LDL,HDL and NEFA in the serum were measured, the myocardial total RNA was extracted by Trizol method, to compare the expression of connexin43 in the following groups, hyperlipemia, normal and drug, we used the technique of RT - PCR, immunostaining and microconfoul, Western blot. RESULTS The concentrations of TC,TG, LDL and NEFA in serum increased（ P 〈 0.05 ） ,while that of HDL decreased（P 〈 0.05）. After treating with allicin, the concentrations of the preceding four indexes was cut down and the con- centrations of the post one went up to nearly normal level. The mRNA expressions and protein expression of eonnexin43 in hyperlipemia group was decreased （ P 〈 0.05 ） , while that of the drug group was enhanced（ P 〈 0.05 ）. We could see the same result in immnnostain- ing. CONCLUSION Hyperlidemic rat model is successfully established, allicin has the effect of lowering blood lipid. Furthermore, the molecular mechanism of allicin is connected with the change of eonnexin43 closely.

关 键 词：大蒜素 高脂血症 间隙连接蛋白43 免疫印迹 免疫组织化学 激光共聚焦 

分 类 号：R96[医药卫生—药理学]