VX-680诱导人膀胱癌T24细胞凋亡及其对Bcl-2表达的影响  

VX-680 induces apoptosis of T24 human bladder cancer cells and down-regulates Bcl-2 expression

作　　者：肖宁[1] 周兴[1] 曾格瓦[1] 赵晓昆[2] 

机构地区：[1]广州医科大学附属第二医院泌尿外科,510260 [2]中南大学湘雅二医院泌尿外科,长沙市410011

出　　处：《实用医学杂志》2014年第7期1060-1062,共3页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2011B031800030)

摘　　要：目的:探讨Aurora A激酶抑制剂VX-680对人膀胱癌T24细胞凋亡的诱导作用及对Bcl-2 mRNA和蛋白表达的影响及意义。方法:体外培养T24细胞,分别给予不同浓度及作用时间的VX-680,Annexin V/PI检测细胞凋亡;Hoechst染色法检测细胞凋亡形态学改变;RT-PCR和Western blot检测细胞Bcl-2 mRNA和蛋白的表达。结果:Annexin V/PI显示,凋亡率分别随着浓度增高和时间增长而增加,呈浓度及时间依赖性;Hoechst染色发现细胞在处理72 h后,其凋亡增多程度及Bcl-2 mRNA和蛋白表达下调水平均随浓度增高而增加,呈浓度依赖性。结论:Aurora激酶抑制剂VX-680可能通过下调Bcl-2表达显著诱导人膀胱癌T24细胞凋亡,且具有浓度依赖性。Objective To investigate the effect of aurora kinase inhibitor VX-680 on cell apoptosis and Bcl-2 expressions in human bladder cancer T24 cells. Methods T24 cells were cultured and treated with VX-680 at various concentrations and time points in vitro. VX-680-induced apoptosis of T24 cells was calculated by flow cytometry. The morphological change of treated cells was observed by microscopy;Bcl-2 mRNA and protein expression in T24 ceils was detected by RT-PCR and Western blot assay, respectively. Results The apoptosis rate of VX-680-induced T24 cells increased in a dose- and time-dependent manner. The increase of apoptosis rate and decrease of Bcl-2 mRNA and protein expression in VX-680-induced T24 cells were in a dose-dependent manner. Conclusion VX-680 can significantly induce the apoptosis of T24 cells by down-regulating Bcl-2expression in a dose-dependent manner.

关 键 词：膀胱肿瘤 VX-680 人膀胱癌T24细胞 细胞凋亡 BCL-2 

分 类 号：R737.14[医药卫生—肿瘤]