PTEN过表达抑制体外活化肝星状细胞Akt的磷酸化  

Overexpression of the tumor suppressor gene PTEN inhibits the phosphorylation of Akt in activated hepatic stellate cells in vitro

作　　者：郝礼森[1] 张晓岚[2] 任昌镇 李立文[1] 王静[1] 莫艳波[1] 边荣荣 魏月[1] 张家琪[1] 刘玉龄 

机构地区：[1]河北联合大学附属医院消化内科,河北省唐山市063000 [2]河北医科大学第二医院消化内科、河北省消化病重点实验室、河北省消化病研究所,石家庄市050000

出　　处：《实用医学杂志》2014年第7期1069-1072,共4页The Journal of Practical Medicine

基　　金：河北省自然科学基金资助项目(编号:H2013209327);中国肝炎防治基金会天晴肝病研究基金资助项目(编号:CFHPC20132078)

摘　　要：目的:探讨过表达的野生型PTEN对体外培养的活化肝星状细胞(HSC)Akt的影响。方法:体外培养活化HSC,以腺病毒为载体将野生型PTEN基因瞬时转染HSC;Western blot及实时荧光定量PCR检测HSC的PTEN、Akt表达;Western blot检测HSC的磷酸化Akt[p-Akt(Thr308)]表达。结果 :携带野生型PTEN基因的腺病毒成功转染HSC,使活化HSC的p-Akt(Thr308)表达下调(P<0.01),而对HSC的总Akt蛋白及mRNA表达没有影响(P>0.50)。结论:过表达的野生型PTEN通过抑制Akt的磷酸化,负性调节活化HSC的PI3K/Akt信号转导。Objective Using an adenoviral vector, the wild-type PTEN gene was transduced into activated hepatic stellate cell （HSC） in vitro and the phosphorylation status of Akt were investigated. Methods The wild type PTEN gene was transduced into activated HSC in vitro mediated by adenoviral vector. The expressions of PTEN and total Akt in HSC were measured by Western blot and Real-time fluorescent quantitation PCR. And the expressions of phosphorylated Akt （Thr308） in HSC was determined by Western blot. Results The data showed that exogenous wild type PTEN gene was successfully transduced and expressed in activated HSC in vitro. The over-expression of wild type PTEN resulted in the significant down-regulated expression of phosphorylated Akt （Thr308） in activated HSC （P 〈 0.01 ）. But no significant defferenees were found in the expression of total Akt in activated HSC at both transcriptional and translational levels（P 〉 0.50）. Conclusions The overexpression of wild-type PTEN can negatively regulate PI3K/Akt signaling transduction by inhibiting the phosphorylation of Akt in activated HSC in vitro.

关 键 词：肝星状细胞 PTEN AKT 信号转导 

分 类 号：R363[医药卫生—病理学]