荧光原位杂交和核基质蛋白22联合检测在膀胱癌诊断中的应用  

Utility of the combination of fluorescence in situ hybridization with nuclear matrix protein 22 in the diagnosis of bladder cancer

作　　者：冉俊武[1] 吕军[1] 王尉[1] 卢玛光[1] 陈晓东[1] 黄晓东[1] 胡卫列[1] 

机构地区：[1]南方医科大学附属临床医学院广州军区广州总医院,广州市510010

出　　处：《实用医学杂志》2014年第7期1073-1076,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金面上项目(编号:81372744);国家自然科学基金青年科学基金资助项目(编号:81001142)

摘　　要：目的:探讨荧光原位杂交和核基质蛋白22对膀胱尿路上皮癌诊断的价值,寻找一种敏感特异的无创性方法诊断膀胱癌。方法:对68例膀胱癌疑似患者的尿液标本同时进行荧光原位杂交、核基质蛋白22、尿脱落细胞学检测,同时,将3种方法结果与组织病理学诊断结果比较,评估它们对膀胱癌的临床诊断价值。结果:FISH、NMP22、尿脱落细胞学的敏感性分别为81.4%、86.0%、39.5%,特异性分别为84.0%、72.0%、96.0%,FISH联合NMP22检测的敏感性为79.1%、特异性为92.0%。结论:FISH-NMP22联合检测有较高的敏感性和特异性,其敏感性远高于细胞学检测,而特异性与细胞学检测相差甚小,联合检测较单一指标的检测对膀胱癌具有更高的诊断价值。Objective To investigate a non-invative, sensitive and specific method to diagnose bladder cancer, and evaluate the value of the combination of FISH with NMP22 in the diagnosis of bladder cancer. Methods Urine from 68 patients suspected suffering from bladder cancer were used by FISH, NMP22 expression and cytologi- cal examination, respectively. The results of above detections were compared to the subsequent histopathology as- say to analyze the specicficity, sensitivity of diagnosis for bladder cancer. Results The sensitivity of FISH, NMP22 expression and the cytological examination was 81.4%, 86.0% and 39.5% respectively, and the specifici- ty of FISH, NMP22 expression and the cytological examination was 84.0%, 72.0% and 96.0%, respectively. The sensitivity and specificity of the combination of FISH with NMP22 was 79.1% and 92.0%. Conclusions The combined diagnosis of FISH and NMP22 for bladder cancer showed greater sensitivity than that of the conventional cytology, with similar specificity as the conventional cytology. The combination of FISH with NMP22 test shows more value for the diagnosis of bladder cancer than any single assay.

关 键 词：膀胱肿瘤 荧光原位杂交 核基质蛋白22 

分 类 号：R737.14[医药卫生—肿瘤]