iTRAQ结合质谱筛选胃癌血清生物标记物  

Screening serum biomarkers in gastric cancer by iTRAQ-Coupled 2D-LC-MS / MS

作　　者：韦春回[1,2] 赖铭裕[1] 莫喜晶[3] 

机构地区：[1]广西医科大学第一附属医院老年消化内科,南宁市530021 [2]广西民族医院消化内科 [3]广西中医药大学第一附属医院仁爱分院,南宁市530001

出　　处：《实用医学杂志》2014年第7期1154-1157,共4页The Journal of Practical Medicine

基　　金：广西科学研究与技术开发计划项目(编号:桂科攻1140003A-13)

摘　　要：目的:利用同位素标记相对和绝对定量(iTRAQ)技术筛选并鉴定胃癌、胃黏膜肠上皮化生患者和健康人血清差异表达蛋白。方法:收集胃癌(GC)、胃黏膜肠上皮化生患者(IM)及正常人血清各45例,组内等量混合后,去除高丰度蛋白,经iTRAQ试剂标记后采用二维液相色谱-串联质谱(2D-LC-MS/MS)鉴定并相对定量。结果:共鉴定出10 540条独特肽段,对应199个非冗余蛋白,相对于正常组,在胃癌组和胃黏膜肠上皮化生组中均表达上调的差异蛋白有23种,下调的有14种,差异蛋白中包括脂多糖结合蛋白(lipopolysaccharide-binding protein,LBP)、C反应蛋白(C-reactive protein,CRP)及无花果酶3(Ficolin-3,FCN3)等9种免疫反应蛋白,提示免疫反应和胃癌间存在一定的联系。结论:筛选出多种与胃癌发生发展的差异蛋白,提示iTRAQ技术对于胃癌蛋白质组学血清生物标记物的研究有很好的应用前景。Objective To apply the Isobaric tags for relative and absolute quantitation （iTRAQ） to identify proteins differentially in serums of gastric cancer （GC） patients, gastric mucosal intestinal metaplasia （IM） patients and normal control population. Methods The study included serum samples from GC group, IM group and normal control group with 45 cases each group, Proteins differentially regulated in serums were identified by the iTRAQ coupled with two-dimensional liquid chromatography/tandem mass spectrometry （2D-LC-MS/MS） technology. Results 10 540 unique peptides, of which correspond to a set of 199 proteins were identified, 23 proteins were over expressed and 14 proteins were underexpressed in GC and IM.Nine immune response proteins such as Lipopolysaccharide-binding protein,C-reactive protein and Ficolin-3 were found in the differential proteins, implying a close linkage between immune response and GC. Conclusions iTRAQ technology may help to identify novel serum markers for early diagnosis of GC.

关 键 词：胃肿瘤 ITRAQ 血清生物标志物 

分 类 号：R735.2[医药卫生—肿瘤]