养分缺乏下外源有机酸对暗棕壤脱氢酶活性的影响  

Effects of Exogenous Organic Acids on Dehydrogenase Activity in Dark Brown Forest Soils under Nutrient Deficiency

作　　者：马瑞[1] 黄文斌[1] 杨迪[1] 刘永 宋金凤[1] 

机构地区：[1]东北林业大学,黑龙江哈尔滨150040 [2]黑龙江出入境检验检疫局技术中心,黑龙江哈尔滨150001

出　　处：《安徽农业科学》2013年第34期13221-13223,13289,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31370613);国家重点基础研究发展计划(973)(2011CB403202);国家质检总局课题(2009IK177);中央高校基本科研业务费专项资金资助项目(DL12CA01)

摘　　要：[目的]研究养分缺乏下外源有机酸对暗棕壤脱氢酶活性的影响。[方法]用不同比例A1和B层暗棕壤(A1∶B=1∶2)设置土壤养分缺乏条件、栽植落叶松幼苗,通过有机酸溶液外源添加,系统研究不同浓度草酸、柠檬酸和琥珀酸对养分缺乏下暗棕壤脱氢酶活性的影响。[结果]养分缺乏下,暗棕壤的脱氢酶活性明显降低,且胁迫时间越长降低幅度越大。对于养分缺乏的暗棕壤,大多数有机酸处理均提高了土壤的脱氢酶活性,提高程度因处理时间、有机酸种类和浓度而异。在处理10、20和30 d时,最显著的有机酸处理浓度分别为10.0、5.0和10.0 mmol/L;不同处理时间内对脱氢酶活性的增幅为30 d>20 d>10 d;3种有机酸的增加效果为:琥珀酸>柠檬酸>草酸。[结论]外源有机酸对养分缺乏暗棕壤的脱氢酶活性提高有很好的促进作用,也一定程度上提高了暗棕壤的微生物活性和土壤肥力。[ Objective] This study aimed to investigate the effects of exogenous organic acids on dehydrogenase activity in dark brown forest soils under nutrient deficiency. [ Method ] Different proportions of A~ and B layer dark brown forest soils （A1： B = 1： 2） were utilized to es- tablish soil nutrient conditions for cultivation of Larix olgensis seedlings. The effects of oxalic acid, citric acid and succinic acid on dehydro- genase activity in dark brown forest soils under nutrient deficiency were studied systematically by adding different concentrations of organic acid solutions. [ Result] Under nutrient deficiency, debydrogenase activity in dark brown forest soils was reduced significantly, and the reduction increased with the extension of stress duration. Most organic acid treatments improved dehydrogenase activity in nutrient-deficient dark brown forest soils, and the effects varied with different treatment durations and types and concentrations of organic acids. On day 10, 20 and 30, 10. 0, 5.0 and 10.0 mmol/L organic acid treatments exhibited the most significant effects, respectively. The increase of dehydrogenase activi- ty in different durations showed a downward trend of 30 d 〉 20 d 〉 10 d; the improvement effects of three organic acids on dehydrogenase ac- tivity showed a downward trend of succinic acid 〉 citric acid 〉 oxalic acid. [ Conclusion] Exogenous organic acids improved significantly de- hydrogenase activity in dark brown forest soils under nutrient deficiency and also improved the microbial activity and soil fertility to a certain extent.

关 键 词：落叶松(Larix olgensis) 养分缺乏 有机酸 暗棕壤 脱氢酶活性 

分 类 号：S714.3[农业科学—林学]