苯胺黑药降解菌降解条件优化及降解酶特性的研究  

Optimization of Degradation Conditions for Degrading Bacteria of Dianilinodithiophosphoric Acid and Properties of Degrading Enzyme

作　　者：宋卫锋[1] 武纯[1] 林丽婷[1] 倪俊[1] 汪卫[1] 

机构地区：[1]广东工业大学环境科学与工程学院,广东广州510006

出　　处：《安徽农业科学》2013年第35期13486-13488,共3页Journal of Anhui Agricultural Sciences

摘　　要：从SBR反应器中分离出一株能高效降解苯胺黑药的菌株AFF0039,并且提取该菌株产生的苯胺黑药降解酶,对降解酶的特性及AFF0039菌株降解苯胺黑药条件优化进行了研究.结果表明,当降解酶为诱导酶,温度在25 ～40℃时,降解酶的热稳定性高,酶活保留率在65％以上;当pH在4.3 ～7时,降解酶的酸碱稳定性高,酶活保留率在85％以上.正交试验优化结果表明,菌株AFF0039降解苯胺黑药最佳条件为时间30h,温度35℃,pH6,此时苯胺黑药降解率达到98.61％;同时,菌株AFF0039产生降解酶的最佳条件为时间12h,温度40℃,pH 5,此时降解酶的活力达到最大值49.01 μg/（h·183;ml）.A strain named AFF0039 with high capacity of degrading dianilinodithiophosphoric acid was isolated from SBR system, and the de- grading enzyme produced by the strain was extracted, properties of the enzyme and optimization of the degrading conditions were studied. The results show that the degrading enzyme is an induced enzyme with high thermal stability at 25 -40 ℃, enzyme activity retention rate above 65% ; at pH4.3 -7, the enzyme shows high stability, enzyme activity retention rate is above 85%. The results of orthogonal test show that the optimal conditions for AFF0039 degrading dianilinodithiophosphoric acid are 30 h, 35 ℃, pH6, the degrading rate achieves 98.61% ; the op- timal conditions for AFF0039 producing degrading enzyme is 12 h, 40 ℃, pH5 ,activity of the degrading enzyme achieves the maximum value 49.01 μg/（h · ml）.

关 键 词：苯胺黑药 降解酶 特性 

分 类 号：X703[环境科学与工程—环境工程]