NDRG2截断肽对胶质瘤细胞系U87MG和U251的抗肿瘤作用  

Anti-tumor effect of the truncated peptides from NDRG2 on U87MG and U251 cells

作　　者：李柏樟 李力[2] 秦晓琴 邓艳春[2] 

机构地区：[1]第四军医大学口腔医学系,陕西西安710002 [2]第四军医大学西京医院神经内科,陕西西安710032 [3]西安市东方医院神经内科,陕西西安710002

出　　处：《中华神经外科疾病研究杂志》2014年第2期118-122,共5页Chinese Journal of Neurosurgical Disease Research

基　　金：陕西省社发攻关基金资助项目(2011K13-01-02)

摘　　要：目的探讨源于N-myc下游调节基因2(NDRG2)蛋白的截短肽对胶质瘤细胞系U87MG和U251的抗肿瘤作用。方法设计、构建人类NDRG2蛋白若干截短肽片段的真核表达载体,转染至上述两种肿瘤细胞中,通过流式细胞术(FCM)观察肿瘤细胞的凋亡和周期阻滞情况。结果流式细胞术结果显示,包含NDRG2蛋白C末端的片段均能不同程度的诱导两种肿瘤细胞系的周期阻滞。结论 NDRG2的C末端是其抗肿瘤作用的关键,很有可能从中筛选出具有治疗意义的抗肿瘤肽。Objective The anti-tumor effect of the tnncated peptides derived from N-myc down stream regalated gene 2 （NDRG2） on U87MG and U251 cells is observed.Methods The hNDRG2 protein truncated into several parts and their eukaryotic expression plasmids were constructed respectively.The two glioma cell lines （U87MG,U251） were transfected by the eukaryotic expression plasmids and their cell cycle arrest and apoptosis were detected by flow cytometry （FCM）.Results The results of flow cytometry showed all the segments which contained the C-terminal of NDRG2 could lead to cell cycle arrest in different degrees extending to all two cell lines.Conclusion C-terminal of NDRG2 plays the most important role in its anti-tumor effect.It is of high probability to find anti-tumor peptides from NDRG2.

关 键 词：N-myc下游调节基因2 截短肽 胶质瘤 

分 类 号：R739.4[医药卫生—肿瘤]