丙酮酸乙酯对人脑胶质母细胞瘤U251细胞和HMGB1的抑制作用  

Inhibition of ethyl pyruvate on human glioblastoma U251 cells and HMGB1

作　　者：李松[1,2] 杭春华[1,2] 苏兴奋 刘明[1,2] 刘寰东 曹立平[1,2] 

机构地区：[1]南方医科大学南京临床医学院 [2]南京军区南京总医院神经外科,江苏南京210002

出　　处：《中华神经外科疾病研究杂志》2014年第2期123-127,共5页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(81171170)

摘　　要：目的探讨丙酮酸乙酯(EP)对人脑胶质母细胞瘤U251细胞的作用及可能的机制。方法不同浓度EP处理U251细胞后,分别用细胞计数试剂盒(CCK-8)和平板克隆的方法检测EP对U251增殖及克隆形成能力的影响,并采用Hoechst 33258染色和膜联蛋白V-异硫氰酸荧光素/碘化丙啶(Annexin V-FITC/PI)染色流式细胞术观察细胞凋亡的变化,蛋白印迹法分析高迁移率族蛋白B1(HMGB1)表达水平的变化。结果 EP(10、15、20 mmol/L)分别作用8 h、16 h、24 h后均能抑制U251细胞的增殖,且存在显著的剂量-时间效应关系。与对照组相比,EP处理后U251细胞克隆形成能力减弱、细胞凋亡率升高。不同浓度组EP作用U251细胞24 h后HMGB1的蛋白表达水平随药物浓度的增加而降低。结论 EP能明显抑制人脑胶质母细胞瘤U251增殖,并诱导其凋亡,其机制可能与下调HMGB1的表达水平有关。Objective The effect and mechanism of ethyl pyruvate （EP） on human glioblastoma U251 cells are investigated.Methods U251 cells were treated with EP at different concentrations,and the effects of EP on cell proliferation and colony formation of U251 cell were measured by cell counting kit-8 （CCK-8） assay and plate colonyforming test,respectively.And the cell apoptosis was detected by Hoechst 33258 staining and flow cytometry with Annexin V-fluorescein isothiocyanate （FITC） and propidium iodide （PI） double staining.The expression of high mobility group box 1 protein （HMGB1） was measured by Western blot.Results EP （10,15,20 mmol/L） inhibited the proliferation of U251 cells in a time-and dose-dependent manner after treated for 8 h,16 h,24 h,respectively.Compared with the control,U251 cells colony formation was decreased after EP treatment and the apoptotic ratio was significantly higher.The expression level of HMGB1 was decreased with the increase of drug concentration after different concentrations of EP treatment for 24 h.Conclusion EP can inhibit the proliferation of human glioblastoma U251 cells and induce their apoptosis by reducing the expression of HMGB1.

关 键 词：丙酮酸乙酯 胶质母细胞瘤 胶质母细胞瘤U251细胞 高迁移率族蛋白B1 

分 类 号：R739[医药卫生—肿瘤]