靶向超声造影剂对卵巢癌新生血管的评价  

Evaluation of Targeted Ultrasoound Contrast Agent on Angiogenesis in Ovarian Cancer

作　　者：杨钦涵[1] 刘慧[1] 向红[1] 

机构地区：[1]新疆医科大学第一附属医院妇产超声科,乌鲁木齐市830054

出　　处：《中国超声医学杂志》2014年第4期368-371,共4页Chinese Journal of Ultrasound in Medicine

基　　金：新疆医科大学第一附属医院科研奖励基金(No.2012YFY26)

摘　　要：目的应用携血管内皮生长因子受体2(VEGFR2)单抗的靶向超声造影剂(MBt),探讨靶向造影剂对裸鼠卵巢癌移植瘤新生血管的应用价值。方法裸鼠卵巢癌SKOV3细胞株皮下移植瘤模型的建立;采用"生物素-亲和素桥连接法"制备MBt;荷瘤鼠分别注射MBt与普通超声造影剂(MBc)行超声造影检查并分析各组时间强度曲线(TIC)相关参数;免疫组化检测肿瘤组织新生血管VEGFR2的表达。结果成功制备携VEGFR2单抗的MBt;MBt组峰值强度较MBc组高(P<0.01),MBt组持续时间较MBc更长(P<0.01);免疫组化示肿瘤组织内新生血管内皮VEGFR2高度表达。结论携VEGFR2单抗MBt能够与裸鼠卵巢癌移植瘤新生血管内皮特异性结合并实现靶向分子超声显像,对卵巢癌新生血管形成的评估提供了新方法。Objective To investigate the applicability of targeted ultrasound contrast agent microbubbles with vascular endothelial growth factor receptor type 2 （MBt） in angiogenesis in ovarian transplantation tumor of nude mice. Methods The transplant tumor model of SKOV3 in nude mice was established and MBt was prepared by biotin- avidin conjugation methods Mice with tumor were treated with MBt and control microbubbles （MBc） for ultrasound contrast examination and relative parameter analysis of time-intensity curves （TIC） ;VEGFR2 expressions in angiogen- esis of tumor tissues were analyzed by immunohistochemistry. Results MBt carrying with VEGFR2 was prepared successfully;MBt group has higher peak intensity （P〈0.01）and longer lasting time （P〈0.01） ; Immunohistochemis- try detected high VEGFR2 expression in angiogenesis endothelium of tumor tissues. Conclusions MBt with VEGFR2 can bind with angiogenesis endothelium in ovarian transplantation tumor of nude mice and achieve targeted ultrasound molecular imaging, it provides a new method on ovarian cancer angiogenesis evaluation.

关 键 词：超声检查 靶向超声造影剂 卵巢癌 肿瘤新生血管 血管内皮生长因子 

分 类 号：R737.31[医药卫生—肿瘤]