小麦新品种“山农20”抗病基因的分子检测  

Resistance Genes of Wheat Variety Shannong 20 Identified by Diagnostic Molecular Markers

作　　者：李继发[1] 邓志英[1] 孙福来 关西贞[1] 王延训[1] 田纪春[1] 

机构地区：[1]山东农业大学作物生物学国家重点实验室/山东省作物生物学重点实验室,山东泰安271018 [2]山东省滨州市种子管理站,山东滨州256600

出　　处：《作物学报》2014年第4期611-621,共11页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31171154);山东省农业良种工程项目(鲁农良种字[2011]7号和[2012]213号);国家转基因生物新品种培育重大专项(2013ZX08002-003)资助

摘　　要：山农20是2011年和2012年分别通过国家黄淮南、北片审定的小麦高产多抗新品种,在国家区试抗病性鉴定和生产中都表现出良好的抗黄淮麦区主要病害的特性。本研究利用与小麦抗白粉病、条锈病、叶锈病、纹枯病基因和抗赤霉病主效QTL紧密连锁的SSR、SCAR、STS等标记对该品种进行了分子检测,发现山农20含有6个抗白粉病基因(Pm12、Pm24、Pm30、Pm31、Pm35和Pm36),6个抗条锈病基因(Yr5、Yr9、Yr15、Yr24、Yr26和YrTp1),2个抗叶锈病基因(Lr21和Lr26),1个抗纹枯病基因(Ses1),但未检测到抗赤霉病主效QTL。分子检测结果部分解释了山农20的优良抗病性,也为利用分子标记辅助选择培育抗病稳产小麦新品种提供参考。Shannong 20 is a newly released winter wheat variety in the National Regional Trials for North and South Yellow-Huai River Valleys, which exhibits excellent resistance to major diseases of wheat. In this study, the possible resistance genes in Shannong 20 were identified by using SSR, SCAR, and STS markers tightly linked to resistance genes/loci against powdery mildew, stripe rust, leaf rust, sheath blight, and scab. A total of 15 resistance genes were detected, including six （Pm12, Pm24, Pm30, Pm31, Pm35, and Pm36） for powdery mildew resistance, six （Yr5, Yr9, Yr15, Yr24, Yr26, and YrTpl） for stripe rust resistance, two （Lr21 and Lr26） for leaf rust resistance, and one （Sesl） for sheath blight resistance. However, no resistance locus to scab was found. The result may partially explain the multiresistance in Shannong 20, and provide some references for cultivating new varieties with disease resistance and stable yield by using molecular marker-assisted selection.

关 键 词：山农20 抗病性相关基因 分子标记 

分 类 号：S512.1[农业科学—作物学]