防腐剂对24h尿蛋白定量检测的影响  

The affect of preservatives in 24-hour urine protein test results

作　　者：王会平[1] 王小亚 李岩[1] 李斌[1] 张惠中[1] 

机构地区：[1]第四军医大学唐都医院检验科,陕西西安710038 [2]渭南市第一医院儿科,陕西渭南714000

出　　处：《国际检验医学杂志》2014年第7期858-859,共2页International Journal of Laboratory Medicine

摘　　要：目的分析防腐剂对24h尿蛋白检测结果的影响,探讨留取24h尿测定尿蛋白加入防腐剂的必要性。方法在同一时间收集健康体检者尿蛋白定性为阴性的尿液后,加入不同量蛋白标准品和大肠杆菌菌液,检测初始和室温保存24h后的尿蛋白水平。采用单因素方差分析探讨3种蛋白浓度梯度下,含不同细菌数的尿液室温保存24h后清蛋白水平变化。结果在保存于室温,不加防腐剂的情况下,不同蛋白浓度且含不同细菌数的尿液蛋白浓度测定值可稳定保持24h,在24h内测定值没有明显改变(P>0.05)。结论用于24h尿蛋白定量检测的标本可以不加入防腐剂。Objective Analysis of the affect of preservatives in 24 hour urine protein test results,and explore the necessity of adding preservatives in 24 hour urine specimens to measure the Urine Protein. Methods After collected the urine which characterized as negative urine protein at the same time, adding different amounts of standard protein and the Escherichia coli bacte- ria,and detecting the urine protein content at initial temperature and after 24 hours at room temperature. Discussed the protein changes of urine under three proteins concentration gradients in the urine after 24 hours at room temperature. Results The urine protein having different protein concentration and containing different number of bacteria in stored at room temperature, without preservatives,could be stably maintained for 24 hours, and the measured value did not change significantly within 24 hours（P〉 0.05）. Conclusion The urine protein specimens for 24-hour quantitative detection does not need to add preservatives.

关 键 词：尿蛋白 24小时尿 保存 防腐剂 

分 类 号：R446.6[医药卫生—诊断学]